Estrogen-induced relaxation of the rat tail artery is attenuated in rats with pulmonary hypertension.
Journal Title: Pharmacological Reports - Year 2010, Vol 62, Issue 1
Abstract
The mechanisms involved in the effects of estrogen on arterial smooth muscle contractility are very complex and not fully clarified. Therefore, the aim of this paper was to examine the mechanisms of estrogen-induced relaxation of the rat tail artery and, specifically, how pulmonary hypertension affects this action. We used male rats and performed experiments on isolated tail arteries in a control group and a group with pulmonary hypertension (PAH) induced by monocrotaline (60 mg/kg b.w. ip). The pD2 value (-log EC50) of phenylephrine significantly decreased in the presence of 20 microM of 17beta-estradiol (5.4 +/- 0.13 vs. 4.9 +/- 0.12, p < 0.05, n = 6). Estrogen-induced relaxation of a phenylephrine-precontracted tail artery has two components: endothelium-dependent (ED) and endothelium-independent (EI). The estrogen effect was independent of ATP-sensitive K+ channels, vasoactive prostanoids and nitric oxide. PAH augmented the maximal effect of phenylephrine on the tail artery contractility but did not affect estrogen-induced ED-relaxation. However, the EI component of relaxation induced by estrogen was completely abolished in tail arteries obtained from animals with pulmonary hypertension. Pulmonary hypertension affects the sensitivity of the rat tail artery to phenylephrine and estrogen, leading to impairment of EI mechanisms of relaxation. Further experiments are required to elucidate the molecular mechanisms of this phenomenon.
Authors and Affiliations
Ivan Kocić, Renata Szczepańska, Iga Wapniarska
Keywords
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