Abstract

Objective: The present study was to evaluate the antibacterial properties of 21 crude extracts from leaf and flower of Aristolochia indica (A. indica), Cassia angustifolia (C.angustifolia), leaf of Catharanthus roseus (C. roseus), Diospyros melanoxylon (D.melanoxylon), Dolichos biflorus (D. biflorus), Gymnema sylvestre (G. sylvestre) and Justicia procumbens (J. procumbens). Methods: The ethyl acetate, acetone and methanol extract of medicinal plants were evaluated against Gram-positive Bacillus cereus (B. cereus) and Gram-negative bacteria Aeromonas hydrophila (A. hydrophila), Enterobacter aerogenes (E. aerogenes), Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) by using well diffusion assay and minimum inhibitory concentration (MIC). Results: The crude plant extracts demonstrated broad spectrum activity against all bacteria. The highest inhibitory zone was observed in leaf methanol extract of A. indica against E. aerogenes (25 mm), and E. coli (20 mm), flower methanol extract of C. angustifolia against B. cereus (22 mm) and leaf acetone extract of G. sylvestre against B. cereus (22mm). The MIC values of leaf methanol extract of A. indica against K. pneumonia (22.6 毺 g/ ml), and flower extract showed against E. coli (MIC: 24.2毺 g/ml), leaf ethyl acetate extract of C. angustifolia against K. pneumoniae (MIC: 28.4 毺 g/ml). Acetone ethyl acetate and methanol extracts of D. melanoxylon and D. biflorus showed the lowest MIC activity value of >30 毺 g/ml against all tested pathogens. Conclusion: The antibacterial activity could be confirmed in most species used in traditional medicine in South India. Nevertheless, traditional knowledge might provide some leads to elucidate potential candidates for future development of new antibiotic agents.

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