Evaluation of antibody against hydatid fluid, protoscolex and adult worms of Echinococcus granulosus antigens by ELISA in mice

Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2009, Vol 1, Issue 1

Abstract

Echinococcosis is one of the world’s relevant zoonoses, affecting both humans and their domestic animals and caused by infection with the metacestode stage of the dog tapeworm, Echinococcus granulosus. In the present study, twenty mice were randomly divided into 4 groups of 5. Each mouse in groups 1, 2 and 3 was immunized with 100 μg of hydatid cyst fluid protein, protoscolex and adult worms of E. granulosus proteins in 100 μl of phosphate-buffered saline which was emulsified with an equal volume of Freund’s complete adjuvant respectively. In group 4, mice were just immunized with above adjuvant in PBS. Mice were boosted four weeks after the first immunization with the same preparation and with Freund’s incomplete adjuvant. Three weeks later, each mouse was challenged with 2000 protoscolices intraperitoneally. Sera were collected before and after each immunization and serum antibodies were tested by ELISA. Results showed that the level of antibody in mice which were immunized with adult worms of E. granulosus on day 28 was roughly seven times higher than before immunization and was higher than hydatid cyst fluid and protoscolex. Level of antibody in mice immunized with adult worms of E. granulosus was also higher than in hydatid cyst fluid and protoscolex groups at day 49. The results of this article indicate that antigen of adult worms of E. granulosus can be used as a candidate for production of a vaccine.

Authors and Affiliations

Gholamreza Hashemi Tabar,Gholamreza Razmi,

Keywords

Related Articles

Therapeutic Effects of ADU-S100 as STING Agonist and CpG ODN1826 as TLR9 Agonist in CT-26 Model of Colon Carcinoma

Cancer immunotherapy emerged as a novel therapeutic approach to destroy tumor cells, and it has grown toward clinical transition following successful fundamental research and clinical trials. Immunotherapy by efficacious...

Effects of the Hydroalcoholic Extract of Peganum harmala Against the Venom of the Iranian Snake Naja naja oxiana in Mice

Peganum harmala contains pharmacologically active compounds and has been utilized for various purposes over the years. Due to public health concerns about snakebite envenoming, this study aimed to assess the potential an...

Preventive Effects of Silymarin on Diclofenac-induced Toxicity in the Domestic Pigeon (Columba livia)

This study aimed to evaluate the effects of silymarin on diclofenac-induced acute liver and kidney poisoning in domestic pigeons (Columba livia). The use of NSAIDs leads to adverse drug effects, such as cardiovascular an...

Monocephalus thoracopagus conjoined twins in a goat

Monocephalus thoracopagus is a kind of attached symmetrical twin in which two nearly complete individuals joined front-to-front in the thoracic region with a single head, fused chests, one or two hearts and two lower b...

Effect of dietary supplementation of garlic and vitamin E on lipid and protein oxidation in common carp meat during different storage times

This study was conducted to investigate the effects of dietary garlic powder and vitamin E supplement for 6 weeks on lipid and protein oxidation markers in nonfrozen and frozen carp meat at various time intervals. Fish w...

Download PDF file
  • EP ID EP722554
  • DOI -
  • Views 56
  • Downloads 1

How To Cite

Gholamreza Hashemi Tabar, Gholamreza Razmi, (2009). Evaluation of antibody against hydatid fluid, protoscolex and adult worms of Echinococcus granulosus antigens by ELISA in mice. Iranian Journal of Veterinary Science and Technology (IJVST), 1(1), -. https://www.europub.co.uk/articles/-A-722554