Evaluation of Decalcified Teeth Sections by Routine Processing & Hot Air Oven Technique
Journal Title: Indian Journal of Dental Advancements - Year 2016, Vol 8, Issue 1
Abstract
Context: Decalcification is the process of removing calcium salts from tissues. Calcified tissue must be decalcified before processing as the tissue cannot be sectioned. Though some agents remove the calcium ions completely and rapidly, they adversely affect the staining characteristics and may also damage the organic components. Aim: The present study was to evaluate the estimation rate of decalcification of four different decalcifying agents and comparision with Hot air oven technique. Materials and methods: Four decalcifying agents namely, neutral ethylene diamine tetra acetic acid (EDTA), nitric acid, formic acid, and combination of formic acid and HCL were used to decalcify 24 natural teeth (three in each solution).The end point of decalcification was evaluated. The decalcified teeth were then routinely processed, sectioned, and stained with hematoxylin and eosin stains. Results: Rate of Decalcification was faster with Hot air oven when compared with Manual procedure among all decalcifying agents. Soft tissue integrity was better with combination of formic acid and HCL as decalcifying agent compared with others. Conclusion: In the present study, it was observed that the rate of decalcification was faster with hot air oven when compared with manual procedure among all decalcifying agents. Regardless of employed technique, soft tissue integrity was better with combination of formic acid and HCL as decalcifying agent compared with others.
Authors and Affiliations
Aruna kumari Maloth, Shyam Prasad Reddy Dorankula, Keerthi Muddana, Pavan G Kulkarni, Nandan SRK
Keywords
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- EP ID EP486255
- DOI 10.5866/2016.8.10010
- Views 104
- Downloads 0
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