Evaluation of in-vitro anthelmintic activity of Heliotropium indicum Linn. leaves in Indian adult earthworm
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 0
Abstract
Objective: To carry out an exhaustive study with a view to substantiate the therapeutic potential of the plant in terms of its anthelmintic activity against Pheretima posthuma using mebendazole as a reference standard. Methods: Forty five worms were collected and were divided into nine groups and were washed in normal saline before they were released into 10 mL of respective drug solutions using distilled water as vehicle. Different concentrations (25 mg/mL, 50 mg/mL and 100 mg/mL) of the test (MEHI and AEHI) and standard solution were prepared before the commencement of the experiment. Time for paralysis and time for death were recorded for each group. Results: Crude methanolic extract with concentrations of 25 mg/mL, 50 mg/mL, 100 mg/mL produced dose-dependent paralysis. Time of paralysis and death increased with concentration of 25 mg/mL, 50 mg/mL as compared with that of standard drug mebendazole. Methanolic extract of Heliotropium indicum Linn. (H. indicum) gave shorter paralysis and death time at 100 mg/mL as compared to aqueous extract H. indicum Linn. Results are expressed as mean依SEM (P<0.05) of 5 worms in each group. Conclusions: From the investigation, conclusion can be drawn that the methanolic extract of H. indicum (Boraginaceae) showed better activity than aqueous extract of the same to treat intestinal worm infections. In comparison with the standard drug mebendazole, methanolic extract of H. indicum showed significant anthelmintic efficacy.
Laboratory evaluation of ethyl acetate and chloroform: methanol (1:1 v/v) extract of Swietenia mahagoni leaf against Japanese Encephalitis vector Culex vishuni group
Objective: To study the larvicidal activity of a furniture tree Swietenia mahagoni L. (S. mahagoni) against mosquito Culex Vishnui group. Methods: Different concentrations of crude, chloroform: methanol (1:1 v/v) and eth...
Evaluation of anti-helmintic activity of Ferula foetida “Hing- A natural Indian spice” aqueous extract
Objective: To evaluate the anti-helmintic activity of Ferula foetida “Hing” against helmintic (Pheretima postuma). Methods: Aqueous extracts from the Ferula foetida resin was investigated for its anthelmintic activity ag...
Correlation between climate variations and rotavirus diarrhea in under-fie children in Bandung
Objective: To find correlations between climate variations (temperature, rainfall, humidity, and wind speed) and the prevalence of rotavirus diarrhea, particularly evinced by hospital admission. Methods: Stool specimens...
A rare cause of cellulitis (necrotic arachnidism): a report of two cases
Bites from brown recluse spiders (Loxosceles reclusa) result in several clinical manifestations, causing painful, disfiguring necrotic ulcers and, uncommonly, severe systemic effects. We report two cases reports with nec...
Single nucleotide polymorphism and expression studies of the interferon gamma gene and its role against Haemonchus contortus in Garole and Sahabadi sheep
Objective: To evaluate the role of interferon gamma (IFN-γ) gene in Haemonchus contortus (H. contortus) resistant in Garole/Sahabadi breed of sheep. Methods: Five Garole sheep (Group I) consistently showing low egg per g...