Evaluation of Leukocyte DNA Damage and Antioxidant Defense in Graves’ Disease; Effect of Medical Treatment
Journal Title: International Journal of Clinical Pharmacology & Toxicology(IJCPT) - Year 2017, Vol 6, Issue 3
Abstract
Background: Oxidative stress has been implicated in many pathological conditions, including hyperthyroidism. In this study, our aim was to investigate the effect of medical treatment on oxidative/antioxidative status and subsequent DNA damage in Graves’ disease, in terms of reduced glutathione (GSH) levels, superoxide dismutase (SOD), glutathione peroxidase (GPx) activity and DNA strand breaks. Methods: Fifty female patients suffering from Graves’ disease and thirty-seven healthy female controls were recruited in the study. Blood samples were taken from the patients before and after treatment. Free T3, free T4 and thyroid stimulating hormone (TSH) levels were determined using chemiluminescent particle assay, GSH, SOD and GPx activity using photometric techniques, and comet assay was utilized to analyze strand breaks with formamidopyrimidine DNA glycosylase (FPG) sensitive sites. Results: Free T3, free T4, GPx activity, DNA strand breaks and FPG sensitive sites were significantly higher, and TSH and GSH levels were significantly lower in patients prior to treatment compared to healthy controls. Following treatment, the levels of free T3, free T4 and TSH were restored to those of healthy controls, but the rise in GSH levels and the drop in GPx activity, strand breaks and FPG sensitive sites were not as profound, though still significantly different than pre treatment levels. Conclusions: The markers of oxidative stress and DNA damage are not restored as quickly as thyroid function tests in Graves’ disease following treatment, suggesting the oxidative damage caused by hyperthyroidism may have long term effects.
Authors and Affiliations
Tulay Akcay
Keywords
Related Articles
- EP ID EP324209
- DOI 10.19070/2167-910X-1700046
- Views 79
- Downloads 0
Population Pharmacokinetic Analysis of Alcohol Concentration after Beer Consumption in Fasted Japanese Subjects
The absorption rate of alcohol is one of the determinants of the peak blood alcohol concentration. Studies focusing on factors that cause inter-individual variability in absorption rate have not been conducted. To conduc...
Efficacy and Safety of Angiotensin-Based Pharmacotherapy versus Conventional Therapy for the Management of Isolated Systolic Hypertension: A Meta-Analysis of Randomized Controlled Trials
The angiotensin (AT) system is highly implicated in the pathophysiology of isolated systolic hypertension (ISH). Consequently, this meta-analysis was designed to quantitatively review the efficacy and safety of angiotens...
Identifying Treatment Response of Sertraline in a Teenager with Selective Mutism using Electrophysiological Neuroimaging
Background: Selective Mutism is described as the inability to verbally express oneself in anxiety provoking social situations and may result in awkward social interactions in school-aged children. In this case-report we...
The Prophylactic and Therapeutic Effects of Safranal and Selenite on Liver Damage Induced by Thyrotoxicosis in Adult Male Rats
Aim: To evaluate the prophylactic and therapeutic activities of safranal and selenite against liver damage induced by thyrotoxicosis. Materials and Methods: Thyrotoxicosis was induced by thyroxine (L-T4, 500 μg/kg, s.c.)...
Steady State Plasma Levels of Bupropion After Administration of 3x150 Mg Extended Release Reference Tablets and Switching to 1x450 Mg Extended Release 450ER Tablets
In order to provide patients who take 450 mg/day bupropion hydrochloride for the treatment of severe depressive disorder with a safe and convenient alternative to currently existing dose regimens, a high-dose bupropion E...