Evaluation of Porcine Gastric Mucin Magnetic Bead Enrichment for the Detection of Norovirus in Bivalve Mollusks
Journal Title: International Journal of Research in Agricultural Sciences - Year 2018, Vol 5, Issue 2
Abstract
Norovirus (NoV) represents one of the leading causes of foodborne illness worldwide. Standard methods for detection or quantification of NoV in bivalve mollusks are now available, but cannot discriminate between infectious and non-infectious virus particles. Based on the ability of intact NoV particle to bind to porcine gastric mucins conjugated to magnetic beads (PGM-MB), this study aimed to develop an enrichment method allowing to assess the infectivity of NoV particles in bivalve mollusks. To achieve this aim, we have investigated the effects of pH of binding buffer, amounts of PGM-MB and the use of additives on the recovery of NoV. The optimal parameters for the capture of NoV in modified Britton–Robinson buffer were pH 6.0; presence of 5% glycerol and 100 µl of PGM-MB. To avoid the negative effect of oyster matrix on the capture of NoV, oyster homogenate needs to be diluted three times in binding buffer. This optimal procedure allowed the detection of 2 × 102 NoV genome copies/ml in clean system and 5 × 103 NoV genome copies/g of oyster digestive tissues. The advantages of this new method are its simplicity and capability of assessing the infectivity of NoV particles.
Authors and Affiliations
Hang Thu Ngo, et al.
Keywords
Related Articles
- EP ID EP501686
- DOI -
- Views 87
- Downloads 0
Evaluation of Best Performing Indigenous Rhizobium Inoculants for Faba Bean Production at Goba and Sinana District of Bale Highland South Eastern Ethiopia
A field trial was conducted for two consecutive cropping season of 2016-2017 (”Bona”) on farmers’ fields at different sites (Sinja & Aloshe) of Goba District and (Jafara & Selka Oda) of Sinana District to evaluate the ef...
Secondary Somatic Embryogenesis for Cork Oak (QuercusSuber L.): Influence of Sugars
This study consists in testing the secondary somatic embryogenesis technique on Moroccan cork oak (Quercus suber L). The experiment was conducted to test particularly the influence of different...
In vitro Shoot Tip Response to Encapsulation-Dehydration Procedure for Cryopreservation of Philippine Taro [Colocasia esculenta (L.) Schott]
This study was conducted to explore the feasibility of the encapsulation-dehydration cryopreservation procedure and provide the groundwork for establishing a cryopreservation protocol for routin...
Transformation of Traditional Fermented Foods of South India
t is understood that traditional fermented foods plays vital role in the life of common people in south Indiaby providingsimple,cheap and healthy food to lead a better life. Introduction of media imposed westernised food...
Role of Forest-Farm Interface Landscape Management Practices on Rural Households Livelihood: the Case of Gurafarda and Arsi-Negele District, Southern Ethiopia
In the Ethiopian context, forest-farm interface landscapes are areas created through encroachment, officially unclassified as either forest or agricultural lands, found under intensive economic activities (crop farmi...