EVALUATION OF TAXIFOLIN AND PHLORETIN AS ANTIANGIOGENIC: AN IN VIVO, IN VITRO EXPERIMENTAL ANALYSIS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: To examine the antiangiogenic effect of flavonoids (taxifolin and phloretin) against various In vivo and In vitro experimental models such as Chick Chorio Allantoic Membrane (CAM) assay, Dorsal Skinfold Chamber (DSFC) and Tube formation assay.Methods: Three different concentrations of drug solution (40µM, 80 µM, 120 µM) and (50 µM, 100 µM, 150 µM) were applied topically during CAM and DSFC assay. These assays were performed to detect antiangiogenic effect of selected molecules. In tube formation assay, Human Umbilical Vein Endothelial Cells (HUVECs) were treated with various concentrations.Results: Antiangiogenic results showed that different concentration of taxifolin and phloretin inhibited new blood vessels and vessels branches per area of CAMs. Similarly, there are reduced numbers and diameter of blood vessels when the doses of the drug increased which are observed in different experimental groups in DSFC model. In vitro anti angiogenic effect of our molecules was further confirmed by inhibition of tube formation on matrigel matrix that involved HUVECs. All the test groups were compared with the control group.Conclusion: The data reported here provide evidence that our flavonoids taxifolin and phloretin revealed prominent anti angiogenic activities.Â
Authors and Affiliations
Md Wasimul Haque, Shakti Prasad Pattanayak, Barij Nyan Sinha
Keywords
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