Evaluation of the cell-free coelomic fluid of the earthworm Eudrilus euginiae to induce apoptosis in SiHa cell line
Journal Title: Journal of Pharmacy Research - Year 2011, Vol 4, Issue 10
Abstract
The cell-free coelomic fluid (CF) was collected from the earthworm Eudrilus euginiae and tested for toxicity at 20, 40, 60, 80 and 100 µl.ml -1 concentrations by MTT assay in SiHa cells invitro. Percentage of inhibition for SiHa cells invitro by CF recorded upto78.52% at 100 µl.ml -1 concentration in 48 hours. AO/EB and Hoechst 33258 staining showed signs of necrosis at 100 µl.ml -1 but morphological changes of typical apoptosis was observed at 20 and 40 µl.ml -1 concentrations. Apoptotic rate increased from 38.58 % to 59.21 %, when repeated at 40 and 50 µl.ml -1 concentrations of CF in 48 hours. Similar increase of apoptotic rate from 68.91% to 79.32% was observed with the increase in the incubation period from 48 h to 72 h suggesting that inducement of apoptosis in SiHa cells invitro by the CF was a function of dose and time period. SDS-PAGE analysis of the CF revealed the presence of a 42 KDa protein – identified earlier to be a tunorilytic factor analogous to the vertebrate cytokine Tumor Necrosis Factor (TNF). Tumorilytic activity of the CF of Eudrilus eugeniae has not been recorded earlier and therefore suggests further investigations in this animal model.
Authors and Affiliations
M. S. Mohamed Jaabi r 1* , L . S h ams h e e r a l i 2 , M. MD. Yasar 1 , S . S e n t h i l Kuma r 1
Keywords
Related Articles
- EP ID EP86645
- DOI -
- Views 127
- Downloads 0
A medicinal plants survey for treatment of obesity
The present day problem right from young people including all the age groups is obesity. It is not just fatness but is the indication of overweight. The plants with several chemical constituents with in themselves act as...
Thevetia peruviana (Pers.) Schum . : A plant with enormous therapeutic potential
An extensive review on the growing requirements, its botany and the medicinal value of this plant has been incorporated. This plant has been known for its diverse range of therapeutic importance in ethno medicine, Ayurv...
Sodium alginate: the wonder polymer for controlled drug delivery
The objective of this paper is to discuss the potential of sodium alginate as a biopolymer in the formulation development and its allied applications. There is a growing trend in pharmaceutical in food industry to avoid...
A Bibliometric research on Medicinal and Aromatic Plants: A Core Journal Study.
The a im of the s tudy i s to eva lua t e ant i s e rotone rgi c a c t ivi ty of e thyl a c e t a t e ext r a c t of Abrus precatorius leaves on a frog fundus strip by using sumatriptan as a standa...
Pharmacophore modeling and 3D QSAR studies on N- (2-benzoylphenyl)-L-tyrosines as PPARg Agonists
Three dimensional pharmacophore modeling studies were performed on a diverse set of N-(2-benzoylphenyl)-L-tyrosine derivatives that demonstrate antidiabetic activity by stimulating peroxisome proliferator activated recep...