Evidence of the association of begomovirus and its betasatellite with the yellow vein disease of an ornamental plant Calendula officinalis (pot marigold) in Rajasthan, India: Molecular, Sequence and Recombination analysis
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2013, Vol 1, Issue 1
Abstract
Begomoviruses are major threats for various crops species throughout the globe, particularly in tropical and sub-tropical regions. Mutation, pseudorecombination and recombination are driving forces for the emergence and evolution of new begomoviruses. In light to that ornamental plants act as an alternate host of begomoviruses and its associated satellite molecules in the absence of main crop. Leaf yellow vein disease of Calendula officinalis plants was observed in Lakshmangarh city of Rajasthan province of India. Samples of Calendula officinalis leaves were collected from the gardens. An expected product of ~550 bp in size was amplified from extracts of symptomatic leaf samples with universal primers on the coat protein region of begomovirus. Moreover, betasatellite were also detected using betasatellite specific universal primers. The presence of begomoviruses was also confirmed by Dot blot hybridization using cloned DNA-A probe of Papaya leaf curl virus. We have identified and characterized the begomovirus and its associated betasatellite through molecular, sequence and recombination analysis. The betasatellite is identified as a new recombinant species, sharing nucleotide identity with other isolates reported from China, Pakistan, Bangladesh and Taiwan. The present study also suggests that the exchange of betasatellites with other begomoviruses would create a new disease complex posing a serious threat to agriculture crops and horticulture ornamental plants production.
Authors and Affiliations
Avinash Marwal, Anurag Kumar Sahu, Rajarshi Kumar Gaur
Keywords
Related Articles
- EP ID EP654363
- DOI 10.24297/jab.v1i1.1554
- Views 229
- Downloads 0
A STUDY ON INVOLVEMENT OF METAL-BINDING PROTEIN(S) FOR THE BIOSORPTION OF SOME SELECTED HEAVY METALS
The present research finding have shown that extracellular protein(s) of growing cells of Pseudomonas aeruginosa DSGPM4 strain are responsible for giving withstand power in presence of toxic heavy metals. To study the ro...
Simulation and Quantification of Tortuous Vessels-Cerebal AVM
A Cerebral Arteriovenous Malformation is an abnormal connection between arteries and veins. In the healthy cases, the blood flows from arteries to veins through capillary bed. But patient having AVM, the capillary bed wi...
Identification of Pathways Associated With Endothelial Dysfunction in Obesity
Obesity has become an increasingly serious health problem and a widespread social focus. It is associated with many diseases, including cardiovascular disease through the induction of endothelial dysfunction. The aim of...
BACTERIAL QUALITY OF DIFFERENT BRANDS OF SACHET WATER SOLD IN FEDERAL UNIVERSITY OF TECHNOLOGY CAMPUS, IMOSTATE, NIGERIA
Drinking water regulations require that potable water for human consumption be free from human disease-causing bacteria and specific indicator bacteria such as coliforms and E. coli. Under total asepsis and using standar...
Effet de l'amendement du substrat dalevage en differentes sources de calcium sur la croissance de Archachatina marginata.
Five hundred and sixty spat of Archachatina marginata (Swainson, 1821), of an average live weight of 2.89 ± 0.13 g with an average shell length of 2.34 ± 0.06 mm and aged around two weeks, were reared for 24 weeks in ten...