Experimental In Vitro Mouse and Human Stem Cell Models for Balanced Activity of Oncogenes and Tumor-Suppressor Genes
Journal Title: Journal of Physiology and Pharmacology Advances - Year 2012, Vol 2, Issue 1
Abstract
Taking in consideration many literature data, according which coordinated oncogenes and tumor-suppressor genes functions is of most importance. In this connection, the main idea was balanced activity of oncogenes and tumor-suppressor genes in vitro in laboratory-cultivated normal cells be achieved. Additional copy of oncogene was inserted Dcn1 in mouse embryonic stem cells (mESCs) by their transfection with appropriate recombinant DNA-constructs. On the other hand, in cultivation of separate sub-populations of non-transfected mESCs in the presence of Doxicyclin, suppressed cell proliferation and increased myeloid cell differentiation was observed, probably by activation of genes from STAT-family. After in vitro-co-cultivation of sub-populations of transfected mESCs, containing additionally-inserted copy of oncogene Dcn1 with sub-populations of non-transfected mESCs, treated with Doxicyclin, the results obtained have indicated the safety and preserved normal/non-tumorigenic cell character of the so derived stem cells in vitro, despite of the presence of additional oncogene copy. These data were confirmed by results from similar experiments with analogical human normal trophoblast cells, immortalized by transfection with SV40 recombinant gene vector.
Authors and Affiliations
Sainova I , Vavrek I , Pavlova V , Daneva T , Iliev I , Yossifova L , Gardeva E , Nikolova E
Keywords
Related Articles
- EP ID EP120038
- DOI -
- Views 106
- Downloads 0
Comparative Study of the Haematology and Serum Biochemistry of Male Wistar Rats Treated with Chloroquine and Artesunate
The comparative effect of Chloroquine and Artesunate; two drugs commonly used in the prophylaxis and chemotherapy of malaria on the haematology and serum biochemistry of male wistar rats was studied. The rats (n=15) weig...
Duloxetine Modulates Vincristine-Induced Painful Neuropathy in Rats
Neuropathic pain is a common and severely disabling state that affects millions of people worldwide. Vincristine-induced peripheral neuropathy models showed mixed sensory motor neuropathy. Duloxetine is the first Food an...
Cathepsin D: A Novel Target for Apoptotic Induction, as a Future Anti-Cancer Therapy: a Review
Among many chemotherapeutic targets, apoptosis is one of the major targets as it reduces the cell mass and thus also control cell proliferation. Apoptosis is strictly regulated through many molecular mechanisms, includin...
Crude Leaf Extracts of Psidium Guajava Could Potentially be Used in Treatment of Type 1 Diabetes
The antidiabetic potential of Psidium guajava ethanolic leaf extract was investigated in Wistar albino rats. Type 1 diabetes model was induced in the rats using Alloxan monohydrate (120 mg/kg) intraperitoneally. The extr...
Carbonyl Protein and Antioxidant Vitamins in Pulmonary and Extrapulmonary Tuberculosis
This Study determines the protein carbonyl cause cellular damage and glutathione, Vitamin A, E, C act as antioxidant. Study carried out in different categories of pulmonary and extra pulmonary tuberculosis of newly sputu...