Expression and Clinical Significance of miR-218 in Colorectal Cancer

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Expression and Clinical Significance of miR-218 in Colorectal Cancer

Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 12, Issue 5

Abstract

Objective: To study expression levels of miR-218 in colorectal cancer and normal tissues. Methods: Using real-time quantitative PCR to detect the expression levels of miR-218 in 60 cases of colorectal cancer and the corresponding normal tissues, calculate its relative expression. Results: The relative expression of miR-218 in the tissues of 60 patients with colorectal cancer was 0.3297±0.2998, which was significantly lower than the control group. The difference expression of miR-218 was statistically significant (P<0.05). There is no difference in the expressing of miR-218 in the gender, age, gross form, pathological type, differentiated degree, lymphatic metastasis, depth of invasion, UICC Stage(P>0.05). There are lower expressions in lymph node metastasis than in those without lymph node metastasis. Conclusion: miR-218 showed low expression in colorectal cancer. Its lower expression levels may be prompted to lymph node metastasis sooner. MiR-218 may act as a tumor suppressor gene involved in tumor development.Colorectal cancer is one of the most common malignant tumors in the digestive tract, ranking 4th in the fatal cause of malignant tumors [1], and its incidence is increasing year by year. Recurrence, metastasis and spread of tumors are still the main causes of death. Therefore, it is of great significance to further study the pathogenesis of colorectal cancer and find a more effective treatment for it. Studies have found that MicroRNAs (miRNAs or miRs) play an important role in cell proliferation, differentiation, apoptosis, gene regulation and tumor occurrence and development. Some miRNAs are involved in the mediation of tumor suppressor genes, while others play a role as oncogenes. Gene chip technology was used to detect the expression profile of miRNAs in various tumor tissue samples, and it was found that most miRNAs were down-regulated in tumor samples and a few miRNAs expression levels were up-regulated. More and more studies have shown that miRNAs have the role of oncogenes or tumor suppressors, and it plays an important role in the process of cell growth, proliferation and apoptosis. The abnormal expression level of miRNAs is related to the occurrence and development of many tumors. Therefore, the study of the relationship between miRNAs and tumor is of great significance for exploring the occurrence, development and treatment of tumors.General Information From June 2016 to June 2018, 60 fresh samples of colorectal cancer that were surgically excised and pathologically confirmed by the affiliated hospital of Inner Mongolia Medical University were collected as the experimental group and the corresponding normal tissue samples as the control group. No preoperative chemotherapy or radiation treatment was performed in all cases, and complete case data was available. Experimental Method: Extraction and Detection of RNA: Tissue samples were taken within 1 h after excision, and quickly placed in a sterile enzymefree cryopreservation tube and stored in liquid nitrogen. Total RNA was extracted by TRIzol reagent. RNA concentration was measured using a DU800 nucleic acid protein analyser (BECKMAN, USA), and RNA integrity was detected by agarose gel electrophoresis.

Authors and Affiliations

Wen Jian Xu, Wei Dong Song, Song Fei, Xiu Lan Su, Liu Ming

Keywords

Colorectal Cancer; miR-218; RT-PCR; iu Ming Colorectal Cancer Tumors Pathogenesis Oncogenes Gene Chip Technology Electrophoresis Amplification Curve Chromosome Heterozygous Deletions Nasopharyngeal Carcinoma

EP ID EP585901
DOI 10.26717/BJSTR.2019.12.002307
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