Extracellular Lipolytic Enzyme Production by a Novel Extremely Halophilic Bacterium
Journal Title: Universal Journal of Environmental Research and Technology - Year 2012, Vol 2, Issue 3
Abstract
Halophilic bacteria live in extreme salt conditions and can produce enzymes which are highly stable. A study was conducted to isolate lipolytic enzyme producing halophilic bacteria from salted fish. As lipolytic enzymes from halophiles can tolerate extreme salt conditions, it is highly stable and can tolerate harsh industrial conditions and thus has got potential biotechnological applications. In the present work 17 halophilic isolates were obtained from salted fish samples on Seghals Gibbons Complex (SGC) medium. The lipolytic activity of the strains was tested by Tween hydrolysis on SGC medium containing 1% Tween 80 or Tween 40. A strain SH1 which showed the highest relative enzyme activity was identified by 16 S rDNA sequencing as a novel strain belonging to the class Gamma proteobacteria. The strain was found to grow in a salt range, 2 to 5 M and thus confirmed as an extreme halophile. It showed lipolytic activity at salt concentration in the range 2 to 4 M and pH range 6 to 11. Thus it forms a promising strain for the production of halophilic lipolytic enzymes and thus can have industrial applications.
Authors and Affiliations
Anisha C, Jyothis Mathew and Radhakrishnan E K
Keywords
Related Articles
- EP ID EP31722
- DOI -
- Views 357
- Downloads 2
Analysis of Mining Operations in the Forest Demarcated as No Go Zones
Forest area in our country is classified into Go Zones and No Go Zones based on their crown density. No Go Zones are dense forest areas where mining operations cannot be permitted. However, for extraction of coal lying...
Analysis of Abiotic Stress Induced Metabolomic Changes in Vigna radiata
The present study involves the use of Phenol, Toluene, Xylene and Hexane as xenobiotics, to induce abiotic stress in mung seedlings under in vitro conditions and to evaluate their impact on seedling’s physiological par...
Production of Vermicompost from Temple Waste (Nirmalya): A Case Study
Vermicomposting is the phenomenon of compost formation by earthworms. Earthworms play an important role in the cycling of plant nutrients, turnover the organic matter and maintain the soil structure. The temple wastes...
Development of Crop Coefficients for Precise Estimation of Evapotranspiration for Mustard in Mid Hill Zone- India
Precise estimation of evapotranspiration obtained by multiplying crop coefficient to reference evapotranspiration using readily available climatic data. Penman-Monteith equation is adopted world wide as the most reliab...
Selection of Sustainable Bivoltine Foundation Crosses to Be Used As Male Components with Multivoltines under West Bengal Conditions
The objective of this breeding study was to develop and identify superior bivoltine breeds possessing the desired targeted traits on productivity along with higher survival under the varied and fluctuation climatic con...