Extraction Optimization for Antioxidant Phenolic Compounds in Elaegnus Angustifolia Using Ultrasound With a Response Surface Methodlogy
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 4
Abstract
Restrictions on the use of synthetic antioxidant such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are being imposed because of their carcinogecity. In this investigation optimization of phenolic extraction process frome methanolic (80%) extract (v/v) of elaegnus angustifolia through Response Surface Methodology by fulin cio- calteu is performrd for optimizing the optimizing the process three factor (time, temperature and solution to solid ratio) were investigated each factor was repeated three times (5,20,35 min), temperature (30, 45,60), and solution to solid ratio (20,35,50). The experiment was conducted based on Box-Behnken design with three factor and three levels comprising a total of 17 tests. During optimization tests in an experiment with extraction conditions as (time:21/66 min, temperature:49/80 and solution to solid ratio: 32/33) the higest phenolic 105/76 mg Gallic acid (standard of phenolic compound) per g of dry material compound was recorded. Considering the results and graphs temperature was indetified as the most effective factor. In addition, the graphs show that increasing behavior of phenolic compound and extract is achieved when time and incremental. In addition to fulin test, DPPH test was also carried out for investigating antioxidant activity. In DPPH test methanolic extract with IC50= 417/74 µg/ml had the highest antioxidant activity.
Authors and Affiliations
Maryam Sardarodiyan| Young Researchers and Elite Club, Quchan Branch, Islamic Azad University, Quchan, Iran, Masoomeh Mehraban Sang Atash| Food Science and Technology Research Institute, ACECR Mashhad Branch, Mashhad, Iran, Akram Arianfar| Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran
Keywords
Related Articles
- EP ID EP13177
- DOI -
- Views 302
- Downloads 12
Functional assessment of CODM gene in different cultivar of Papaveraceous species via in silico analysis
Medicinal use of the opium poppy (Papaver somniferum L) has ancient history, but the isolation of morphine was not described until the early nineteenth century. Morphine is the most important alkaloid of opium poppy in...
Changes in Carbohydrates and Sugar Yield in Sugar Beet (Beta Vulgaris L.) Under Different Biofertilizers and Irrigation Closed Time
Objective: Many agronomic practices may need to be adjusted to maximize yield and quality of sugar beet. Thus agronomic package must be always modified. This sutdy was laid out for evaluation of changes in carbohydrate...
Modeling of Climate Effects on Sugar Beet Growth in Kurdistan Province
Climate is one of the most important factors that influence the quality and quantity of crops. Understanding the natural features of any region, especially about the climate, can play a major role in making plans and lan...
Effects of zinc sulphate and Ascorbic acid on Flowering Characteristics of Ornamental plant Gazania (Gazania rigens) cv. daybreak red stripe
A pot experiment was conducted in a completely randomized design, to study the effect of zinc sulphate (ZnSO4) and Ascorbic acid (AS) foliar application application 20 and 40 ppm was compared to the control (distillate...
Rubia tinctorum L. (Rubiaceae) or Madder as one of the living color to dyeing wool
The medicinal part of Rubia tinctorum is the dried root. The small yellowishgreen flowers are in loose, leafy, long-peduncled terminal or auxiliary cymes. The margin of the calyx is indistinct, 4- to 5-sectioned and ha...