Fabrication and Appraisal of Poly (Lactic-Co-Glycolic Acid) – Moxifloxacin Nanoparticles using Vitamin E-TPGS: A Potential Intracanal Drug Delivery Agent
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 6
Abstract
Introduction: Nanotechnology is gaining momentum in the search for ideal biomaterials by dental researchers. The expansible nature of Endodontology deems exploration, learning and scrutinizing newer avenues which have the potential to be applied and investigated. The popularity of polymers as drug delivery vehicles has opened avenues for their application in the root canal system. This study utilized application of biodegradable polymers as potential drug delivery vehicles against Enterococcus faecalis, one of the main reasons for post treatment disease. Aim: This study aimed at fabricating Poly (Lactic-co-Glycolic Acid) (PLGA)-moxifloxacin nanoparticles and assessing its sustained antimicrobial efficacy with calcium hydroxide and chitosan-moxifloxacin hydrogel against Enterococcus faecalis. Materials and Methods: PLGA (50:50) in a quantity of 100 mg was dissolved in 1 ml ethyl acetate and 45 ml of 0.3% w/v Vitamin-E Polyethylene Glycol 1000 Succinate (vitamin E-TPGS) was kept for magnetic stirring in separate beaker. Moxifloxacin (50 μl) was added to polymer PLGA following which vitamin E-TPGS was added to the polymer. Nanoparticles were fabricated using ultrasonication and collected by centrifugation. Surface characterization was assessed using scanning electron microscope. Results were obtained in the form of zone of inhibition by the nanoparticles against Enterococcus faecalis and comparisons were made with chitosan-moxifloxacin hydrogel and calcium hydroxide using Analysis of Variance (ANOVA) followed by Student t-test. Results: Upon statistical analysis, the zone of inhibition against E. faecalis remained constant with PLGA-moxifloxacin nanoparticles for 14 days while it decreased with chitosan-moxifloxacin hydrogel and remained nil for calcium hydroxide (p<0.01). Conclusion: The study posits that PLGA encapsulated moxifloxacin nanoparticles showcased sustained antibacterial effect in low doses against the test pathogen. Its sustained and programmed release makes them unique contenders for further evaluation in Endodontics as potential intracanal medicaments.
Authors and Affiliations
hardik makkar, gaurav patri
Keywords
Related Articles
- EP ID EP352052
- DOI 10.7860/JCDR/2017/27633.9957
- Views 97
- Downloads 0
Chronic Ingestion of Bisphenol A and Bisphenol S Attenuated Contractile Activity of Rat Stomach and Small Intestine In-vitro
ABSTRACT Introduction: Bisphenol A (BPA) is a chemical used in synthesis of plastic. BPA seeps into food from the plastic containers used to store food items. Exposure to BPA is implicated as a causative factor for detri...
Ankylosing Spondylitis Causing Obstructive Lung Disease and Tricuspid Insufficiency: A Case Report
ABSTRACT Ankylosing Spondylitis (AS) is a chronic progressive inflammatory spondyloarthropathy typically involving the axial skeleton, especially the sacroiliac joints and spine. Genetic predisposition in AS leads to its...
Bone Turnover Markers: An Emerging Tool to Detect Primary Osteoporosis
ABSTRACT Introduction: Osteoporosis is a global health problem, asymptomatic and noticed only after a fracture. Ten years after the cessation of ovarian function due to hormonal imbalance, women show an accelerated phase...
Light Emitting Diode Lighting Flicker, its Impact on Health and the Need to Minimise it
ABSTRACT The existing technologies in Light Emitting Diode (LED) lighting sometimes cause flicker at frequencies that may have adverse health effects. Although, not always obvious to the human eye, it can make a differen...
Determination of Range of Quadriceps Angle by Computed Tomography
ABSTRACT Introduction: The Quadriceps angle (Q angle) has come to be accepted as an important factor in assessing knee joint function. Various conditions can affect the Q angle such as increased pelvic width, sociologica...