Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts
Journal Title: Journal of Research in Biology - Year 2014, Vol 4, Issue 8
Abstract
Ultraviolet B (UVB 290-320 nm) participate in the development of the cutaneous inflammatory response which includes a cascade of events that involves increased expression of cyclooxygenase-2 (COX-2), release of tumor necrosis factor-alpha (TNF-α) and other inflammatory cytokines. Peroxisome proliferator-activated receptors (PPARα/γ) are considered to be potential targets for photo protection because they inhibit UVB mediated inflammatory responses. In this study, we investigated the effect of ferulic acid on UVB-radiation induced expression of TNF-α and COX-2 in human dermal fibroblasts (HDFa). Further, the action of ferulic acid on PPARα/γ activation and its binding interaction with these proteins were analyzed by induced fit docking. We found that onetime UVB exposure (19.8 mJ/cm2) showed significantly increased the expressions of COX-2 and TNF-α in HDFa after 4 h post-irradiation when compared to the control cells. Ferulic acid pretreatment for 30 min before UVB exposure prevented UVB-induced overexpression of these inflammatory markers. It has also been found that ferulic acid activates PPARα/γ expressions in HDFa. Further, induced fit docking analysis showed that there was a greater binding interaction of ferulic acid with PPARγ than PPARα. Thus, ferulic acid exhibits beneficial effects against UVB-induced inflammatory responses probably through down-regulating COX-2 and TNF-α expressions and activating PPARα/γ agonists
Authors and Affiliations
Kanagalakshmi A, Agilan B, Mohana S, Ananthakrishnan D, Velmurugan D, Karthikeyan R, Ganesan M, Srithar G, Rajendra Prasad N
Keywords
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