First phylogenetic analysis of Ehrlichia canis in dogs and ticks from Mexico. Preliminary study
Journal Title: Revista MVZ Cordoba - Year 2016, Vol 21, Issue 3
Abstract
Objective. Phylogenetic characterization of Ehrlichia canis in dogs naturally infected and ticks, diagnosed by PCR and sequencing of 16SrRNA gene; compare different isolates found in American countries. Materials and methods. Were collected Blood samples from 139 dogs with suggestive clinical manifestations of this disease and they were infested with ticks; part of 16SrRNA gene was sequenced and aligned, with 17 sequences reported in American countries. Two phylogenetic trees were constructed using the Maximum likelihood method, and Maximum parsimony. Results. They were positive to E. canis 25/139 (18.0%) dogs and 29/139 (20.9%) ticks. The clinical manifestations presented were fever, fatigue, depression and vomiting. Rhipicephalus sanguineus Dermacentor variabilis and Haemaphysalis leporis-palustris ticks were positive for E. canis. Phylogenetic analysis showed that the sequences of dogs and ticks in Mexico form a third group diverging of sequences from South America and USA. Conclusions. This is the first phylogenetic analysis of E. canis in Mexico. There are differences in the sequences of Mexico with those reported in South America and USA. This research lays the foundation for further study of genetic variability.
Authors and Affiliations
Carolina G. Sosa-Gutiérrez, Ph. D *| Laboratorio Nacional de Genómica y Salud. Texcaltitla, Km 67.5. Singuilucan, Hidalgo. México.Correspondence: mcarososagtz@yahoo.com.mx, Teresa Quintero-Martinez, Ph. D| Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia. Ciudad Universitaria, S/N. México D.F, México., Margarita Vargas-Sandoval, Ph. D| Universidad Michoacana de San Nicolás de Hidalgo, Facultad de Agrobiología, Departamento de Entomología. Av. Lázaro Cárdenas S/N, Col. Revolución, C.P. 60150, Uruapan, Michoacán. México., Guadalupe Gordillo-Pérez, Ph. D| Instituto Mexicano del Seguro Social. Centro Médico Nacional Siglo XXI. Hospital de Pediatría, Unidad de Investigación de Enfermedades Infecciosas y Parasitarias. Cuauhtémoc, Nro. 330, Col. Doctores. México D.F, México.
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