Frequency and antibiogram pattern of gram positive cocci in catheter related blood stream infections (CRBSI) in a tertiary care hospital, Tamilnadu
Journal Title: Pathology Update: Tropical Journal of Pathology and Microbiology - Year 2017, Vol 3, Issue 2
Abstract
Introduction: Blood stream infection associated with catheter is life threatening. Various bacterial agents are responsible for this and Coagulase Negative Staphylococci (CoNS) is now becoming one of the major agent in Catheter Related Blood Stream infection (CRBSI). Objectives: To know the bacterial isolates and the frequency of the CoNS & its antibiotic resistance patterns of in Catheter Related Blood Stream infection (CRBSI). Methods: Catheter tips (5 cm tip) were collected and 5 ml of peripheral blood sample was collected with proper aseptic precautions. Catheter tips were processed using Maki DG et al procedure. Blood was inoculated into the BACTEC blood culture bottle (BacT/ALERT 3D automated blood culture system –BioMerieux). All the gram positive bacteria were further processed for standard biochemical tests. All these isolates were subjected to antibiotic susceptibility testing (Hi-Media discs) by Kirby Bauer disc diffusion test according to CLSI guidelines. Results: Among the total 158 isolates, 55 (34.8%) were gram positive cocci. Coagulase Negative Staphylococci (CoNS), were the predominant isolate in this study. Highest resistance was observed with ampicillin, penicillin, amoxyclav, erythromycin and tetracycline. All the gram positive isolates were 100% sensitive to Vancomycin and Linezolid. Conclusion: Among the total 158 isolates, 55 (34.8%) were gram positive cocci. Coagulase negative staphylococci (CONS) which account 18.98%, followed by 8.86%, Staphylococcus aureus, 5.06% Enterococci and 1.89% were Micrococci. All our isolates were sensitive to Vancomycin and linezolid.
Authors and Affiliations
Rajesh J. G, Senthamarai S, Sivasankari S, Anitha C, Somasunder V, Akila Akila, Kumuthavathi Kumuthavathi
Keywords
Related Articles
- EP ID EP263196
- DOI 10.17511/jopm.2017.i2.04
- Views 134
- Downloads 0
Carrier status of Acinetobacter among healthcare personnel and prevalence of the same in the environment
Background: Acinetobacter baumanii is an increasingly troublesome nosocomial pathogen, often with multidrug and multi resistant strains, mainly in intensive care units (ICUs), being responsible for different types of nos...
Pap smear as early diagnostic tool for cervical cancer- A life saviour
Background: Cervical cancer is a leading cause of mortality and morbidity among women globally and most common gynaecological cancer in developing countries. Papanicolaou smear study is a simple and cost effective screen...
Ki67 expression in triple negative breast cancer: Correlation of Ki67 expression with other prognostic factors in breast cancer in Indian patients
Introduction: Triple negative breast cancer ( TNBC) cases are not sensitive to hormonal therapy and till date no specific targeted medication has been found for TNBC. The present study aims to know the expression of Ki67...
Significance of platelet indices in burns patient
Introduction: Burns constitute an important health concern all over the world. The main aim of burns treatment is reducing morbidity and mortality. Platelet count was proved to be an important prognostic index in burns....
Bactericidal activity of acetone extract of Alpinia galanga on multidrug resistant clinical isolates of Enterococcus faecalis
Introduction: The objective of the study was to evaluate the inhibitory activity of five traditional Indian medicinal herbs against multidrug resistant clinical isolates of E. faecalis. Methods: Sixty five E. faecalisder...