Frequency of Weak D alleles in Blood Donors by Molecular methods
Journal Title: Khoon - Year 2018, Vol 15, Issue 2
Abstract
Frequency of Weak D alleles in Blood Donors by Molecular methods Daneshvar Z.1, Amirizadeh N.1, Oodi A.1, Goudarzi S.1 1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Abstract Background and Objectives Detection of weak D blood type can lead to the saving of RhD negative blood components and can reduce RhIG consumption. Therefore, determining the frequency of weak D alleles in our country is necessary. Materials and Methods In this descriptive study, a total of 105 blood samples with weak expression of D antigen from 21 provinces among Iranian blood donors were studied. The phenotype of samples was tested for Rh D, C, c, E and e antigens by routine serological methods. Weak D variants were evaluated by PCR-SSP, RFLP and DNA sequencing methods. Results Among 105 weak D samples, 9 weak D alleles and 2 partial D alleles were found. The most prevalent weak D type in our population was weak D type 15(38.1%), weak D Type 1(10.48%), Type 5(7.62%), Type 4 (4.76%), Type 80 (3.8%), Type 3 (1.9%) and Type 11 , Type 8 , Type 100 each (0.95%) and Partial D DLO (8.57%), and partial D V a /DAU (0.95%). We also observed the correlation between weak D type 15 and E antigen and weak D type 1 and Partial D DLO and C antigen. Conclusions There is a significant difference between the prevalence of Weak D variants among our blood donors compared to European and American white populations with a high incidence of weak D 1, 2 and 3, equally compared to African population with a high incidence of Partial D and weak D type 4, as well as Asian population with a high prevalence of Del. Key words: Blood Donors, Genotype, Alleles Received: 31 Dec 2017 Accepted: 30 Jan 2018 Correspondence: Amirizadeh N., PhD of Hematology and Blood Banking. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine. P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601599; Fax : (+9821) 88601599 E-mail: n.amirizadeh@ibto.ir Correspondence: Oodi A., PhD of Hematology and Blood Banking. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine. P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601599; Fax : (+9821) 88601599 E-mail: ar.oody@gmail.com
Authors and Affiliations
Z. Daneshvar, N. Amirizadeh, A. Oodi, S. Goudarzi
Keywords
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