Functional, Histopathological and Immunohistichemical Assessments of Cyclosporine A on Sciatic Nerve Regeneration Using Allografts: A Rat Sciatic Nerve Model
Journal Title: Bulletin of Emergency And Trauma - Year 2017, Vol 5, Issue 3
Abstract
Objectives: To study the functional, histopathological and immunohistochemical effect of cyclosporine A on sciatic nerve regeneration using allografts in a rat sciatic nerve model. Methods: Thirty male white Wistar rats were divided into three experimental groups (n = 10), randomly: Normal control group (NC), allograft group (ALLO), CsA treated group (ALLO/ CsA). In NC group left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis muscle was sutured. In the ALLO group the left sciatic nerve was exposed through a gluteal muscle incision and transected proximal to the tibio-peroneal bifurcation where a 10 mm segment was excised. The same procedure was performed in the ALLO/ CsA group and the animals were treated with interaperitoneal administration of cyclosporine A. The harvested nerves of the rats of ALLO group were served as allograft for ALLO/ CsA group and vice versa. The NC and ALLO groups received 300 μL sterile olive oil interaperitoneally once a day for one week and the ALLO/ CsA group received 300 μL CsA (1mg/kg/day) interaperitoneally once a day for one week. Results: Behavioral, functional, biomechanical and gastrocnemius muscle mass showed earlier regeneration of axons in ALLO/ CsA than in ALLO group (p=0.001). Histomorphometic and immunohistochemical studies also showed earlier regeneration of axons in ALLO/ CsA than in ALLO group (p=0.034). Conclusion: Administration of CsA could accelerate functional recovery after nerve allografting in sciatic nerve. It may have clinical implications for the surgical management of patients after nerve transection in emergency conditions.
Authors and Affiliations
Amir Amniattalab, Rahim Mohammadi
Keywords
Related Articles
- EP ID EP297945
- DOI -
- Views 70
- Downloads 0
Effect of Platelet Rich Plasma Combined with Chitosan Biodegradable Film on Full-Thickness Wound Healing in Rat Model
Objective:To assess the effects of platelet rich plasma (PRP) with chitosan biodegradable film on full thickness wound healing in rat. Methods:This was an experimental study being performed in 2015 during a 4-month perio...
Letter to the Editor Regarding “Tips on Reporting Systematic Reviews”
Dear Editor, In response to the letter, We are grateful to reader of paper for his interesting correspondence on our paper [1]. Actually in our paper we focused on generated framework rather than a review study. We syste...
Efficacy and Safety of Morphine and Low Dose Ketamine for Pain Control of Patients with Long Bone Fractures: A Randomized, Double-Blind, Clinical Trial
Objective: To compare the effects of intravenous morphine and a low dose of ketamine on pain intensity of patients with traumatic fractures of the long bones. Methods: This randomized, controlled, double-blinded, clinica...
Effects of Chitosan-Zinc Oxide Nanocomposite Conduit on Transected Sciatic Nerve: An Animal Model Study
Objective : To determine the effects of chitosan-zinc oxide nanocomposite conduit on transected sciatic nerve in animal model of rat. Methods: Sixty male White Wistar rats were used in this study. A 10-mm sciatic nerve d...
Current Concepts and Advancements in Management of Traumatic Brain Injury; A Glimpse at the Recently Published Evidence
The traumatic brain injury (TBI) remains the leading cause of mortality and morbidity in trauma associated injuries worldwide [1]. Although it has been well demonstrated that the epidemiology of the TBI is changing patte...