Gamma irradiation effect on the growth of Musa cv. Tanduk (AAB)
Journal Title: Asian Journal of Agriculture and Biology - Year 2018, Vol 6, Issue 2
Abstract
Improvement of banana through conventional method have been very difficult due to ploidy and sterility of most popular cultivars. Hence, given the difficulties in conventional breeding, mutation breeding was attempted to induce variability and obtain improved banana varieties. Therefore, banana cv. Tanduk shoots meristem (1.5 x 1.0 cm) were exposed to γ rays at doses ranging from 10-70 Gy. Subculture was conducted up to four cycle followed by induction of rooting. The radio-sensitivity of in vitro shoots towards radiation was assessed through the percentage of the explants that survived the treatment. The highest survival rate (74%) among γ treated explants recorded was in 10 Gy treatment and the lowest survivality (20%) was in 70 Gy. The lethal dose (LD50) which had caused 50% mortality to the irradiated material was found to be 37 Gy. The result on shoot growth showed that the highest shoot number (5) per explant recorded at radiation dosage of 20 Gy, followed by 3 shoots per explant at a dosage of 10 Gy and highest shoot length (3.43 cm) was observed at 10 Gy followed by 20 Gy and 30 Gy treatment caused significant reduction in shoot length. Similarly, 10 Gy induced maximum root length, whereas explants irradiated with 20 Gy and 30 Gy caused the reduction in root length significantly. Gamma rays at the lower dosage of 10 and 20 Gy imposed a significant impact on shoot growth and 30 Gy caused reduction in growth. Hence, the present research was conducted to study the effect of γ radiation on banana plant, while observing growth traits in γ irradiated explants.
Authors and Affiliations
Ferid Abdulhafiz
Keywords
Related Articles
- EP ID EP603390
- DOI -
- Views 81
- Downloads 0
Sensitivity of legumes and soil microorganisms to residue of herbicide mixture of atrazine and mesotrione
Mixing of herbicide compounds in one formulation is one way to prevent weed resistance, but it may increase herbicide persistence in the soil and cause eco-toxicity. The research was aimed to evaluate the sensitivity of...
Combined application of sorghum and mulberry water extracts is effective and economical way for weed management in wheat
Wheat (Triticum aestivum L.) is of prime importance being staple food of the masses of Pakistan. Weed infestation in wheat not only reduce the yield but also affects the efficiency of other production factors. Allelopath...
Uptake, Translocation of Pb and Chlorophyll Contents of Oryza Sativa as Influenced by Soil-Applied Amendments under Normal and Salt-Affected Pb-Spiked Soil Conditions
Heavy metal contamination of the soil environment has become a major source of concern and has posed serious human health related problems in many developing countries particularly Pakistan. Chemical immobilization of he...
Morphological, phytochemical and antifungal analysis of Aloe vera L. leaf extracts
This study was undertaken with the aim of analysing some morphological parameters, screening of important phytochemical compounds and the activity of Aloe vera leaf extracts against two fungal species viz; Aspergillus ni...
Effectiveness of multi-strain biofertilizer in combination with organic sources for improving the productivity of Chickpea in Drought Ecology
The present study was conducted in Bahawalpur, which is characterized by extremely dry and harsh desert conditions with very little rainfall and water availability. Due to less availability of water, the production of fo...