General Purpose Media (BNO) for Growing Fastidious Gram Negative (FGN) Bacteria
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2017, Vol 22, Issue 6
Abstract
General purpose media for growing fastidious pathogenic Gram negative (FGN) bacteria are limited to non-selective blood and chocolate agar. These suffer from excessive contamination with fungi, Gram positive, and Gram negative bacteria, especially Pseudomonas spp. MacConkey agar can be modified to grow Haemophilus, Helicobacter, Campylobacter and other FGN without the use of antibiotics. BNO100 and BNO70 media were based on MacConkey medium. BNO1-4 were Blood Agar Base containing crystal violet and no bile salts. All media were made as chocolate agar with 8% defibrinated sheep blood, yeast extract 0.5%, glycerol 0.5% (v/v), vitamins, and agar. Campylobacter jejuni ATCC29428 and Haemophilus influenzae were able to grow on all described media including BNO100. Helicobacter pylori ATCC43504 grew on all BNO media but not BNO100. Most BNO media were inhibitory for the growth of several Gram positive bacteria; Streptococcus pneumoniae, Staphylococcus epidermidis, Bacillus atrophaeus QUBC16, Staphylococcus aureus including three MRSA clinical isolates. C. jejuni and H. pylori were successfully grown on BNO media containing Dent’s, Skirrow’s, or Line’s selective antibiotic-supplements. MacConkey based BNO Chocolate agar media may be used as general purpose selective media for Gram negative bacteria including FGNs. We recommend the utilization of BNO70 and BNO2 for research and clinical sample processing involving FGN bacteria. The antibiotic-free BNO media reduced contamination with Gram positive and fungi. The incorporation of selective antibiotics and antifungal agents was compatible with the formulated BNO media; whereas Pseudomonas continued to pause a serious contamination problem.
Authors and Affiliations
Abdallah Bani Odeh, Issa Shtayeh, Mohammed Ayesh, Ismail Warad, Sameer A. Barghouthi
Keywords
Related Articles
- EP ID EP311930
- DOI 10.9734/JAMMR/2017/34450
- Views 99
- Downloads 0
In-vitro Shear Bond Strength of a Nano-composite Resin in Comparison with Microfilled and Microhybrid Composite Resins
Background: Nanotechnology has revolutionized all aspects of research and development, and dentistry has not been an exception. Nanocomposites are a gift of this nanotechnology. Extensive work has been done on its variou...
Parallel Screening with Papanicolaou Smear and Visual Inspection Tests Significantly Reduced False Negative Screening Results in Southern Brazil
Aims: To verify the screening performance of Pap smear and four modalities of visual inspection tests (VIT) in screening for cervical intraepithelial neoplasia CIN of grade 2, 3 or carcinoma in situ, in a low risk popula...
Complicated Fracture and Resorption of an Osteochondroma
Aims: To describe two unusual complications of a typical solitary pedunculated femoral osteochondroma that included a distal fracture of the exostosis and a missed fragment following surgical treatment as well as to eval...
A Simple Computed Tomography Scoring System to Predict Histological Malignancy of Solitary Fibrous Tumors of the Pleura
Purpose: The aim of the present study was to define the very first score enabling discrimination between benign and malignant solitary fibrous tumors of the pleura (SFTPs), on the basis of reliable preoperative CT featur...
The Effect of Geothermal Water on Skin Condition and Body Fat
Balneotherapy for therapeutic purposes has been used since ancient times, and was based on empirical experience carried forward from generation to generation. There is scientific evidence about the positive effects of th...