Genetic diversity and phylogeny of alfalfa nodulating rhizobia assessed by nifH and nodA genes
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2012, Vol 3, Issue 7
Abstract
The alfalfa (Medicago sativa L.) is broadly cultivated in various regions of Iran, but information on the genetic structure and phylogeny of the rhizobia nodulating this legume crop is scarce. The genetic diversity and phylogeny of 16 strains, isolated from root nodules of alfalfa was assessed by nifH and nodA genes sequencing. Results showed that, nifH and nodA fragments for Six strains could not be amplified and There were no nodules obtained from the plants inoculated with these strains as well. These findings suggest that they may be non-symbiotic and opportunistic bacteria that occupy root nodules. Based on the sequences of nodA and nifH genes, ten strains belonged to the S. meliloti.
Authors and Affiliations
A. A. Soltani Toolarood*| Department of soil science, college of agriculture, University of Tehran, Karaj, Iran,ali_soltani_t@yahoo.com, H. A. Alikhani| Department of soil science, college of agriculture, University of Tehran, Karaj, Iran, Gh. Salehi| Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran, H. Asadi-Rahmani| Department of Soil Microbiology, Soil and Water Research Institute, Tehran, Iran, K. Khavazi| Department of Soil Microbiology, Soil and Water Research Institute, Tehran, Iran, A. A. Poorbabaee| Department of soil science, college of agriculture, University of Tehran, Karaj, Iran, K. Lindström| Department of Applied Chemistry and Microbiology, Viikki Biocenter, University of Helsinki, Finland
Keywords
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