Genexpert® MTB/RIF for Rapid Identification of Clinically Suspected MDR-TB Cases
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 3
Abstract
Introduction: An alarming increase in global incidence of drug-resistant Mycobacterium tuberculosis infection has created a critical need for methods that can rapidly detect it. Multidrug-resistant tuberculosis is defined as tuberculosis caused by M. tuberculosis that are resistant to at least isoniazid and rifampicin (RIF). The process to detect M. tuberculosis is time consuming. GeneXpert® MTB/RIF detects it within two hours. Aim and Objectives: The study was undertaken to detect M. tuberculosis and simultaneously RIF resistance in clinically suspected MDR cases of pulmonary TB by using GeneXpert® MTB/RIF. Compare MTB detection with sputum smear status and geographical mapping of clinically suspected MDR-TB cases and confirmed by GeneXpert® MTB/RIF. Material & Methods: Sputum samples from 100 clinically suspected MDR-TB cases were subjected to sputum smear microscopy followed by to GeneXpert® MTB/RIF. Results: Amongst 100 clinically suspected MDR-TB cases, 81 (81%) were smear-positive and 19 (19%) smear-negative. M. tuberculosis was detected amongst 69 (85.19%) smear-positive and 6 (35.29%) smear-negative cases. Amongst 75 MTB detected cases, 58 (77.33%) were RIF sensitive, 12 (16%) resistant and 5 (6.67%) indeterminate. Discussion: In100 clinically suspected MDR-TB cases, only 12 (16%) showed RIF resistance. The MDR-TB appears to be low in our area against clinically suspected MDR cases. This may be due to irregular, inadequate and intermittent treatment leading to clinical failure of primary line anti-TB drugs. Emphasis on proper counselling is needed while starting primary line anti-TB drugs. Conclusion: GeneXpert®MTB/RIF assay may prove to be useful in rapid diagnosis and initial management of clinically suspected MDR-TB cases.
Authors and Affiliations
Shevade Smita Uday
Keywords
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