GENOTYPE AND VIRAL LOAD DETERMINATION OF HEPATITIS C VIRUS FROM A TERTIARY CARE HOSPITAL, SOUTH INDIA
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2019, Vol 8, Issue 1
Abstract
BACKGROUND Hepatitis C Virus (HCV) is a leading cause of chronic liver disease which frequently progresses to cirrhosis and hepatocellular carcinoma. Genotype distribution of HCV in a geographical area and pre-treatment viral load are the prognostic markers of response to antiviral therapy which may help to decide the duration of treatment and to develop geographically tailored vaccine immunogens. MATERIALS AND METHODS A descriptive study with qualitative detection of anti-HCV in patients’ serum was carried out by Chemiluminescent Immunoassay (CLIA) in VITROS. Samples positive in CLIA were further processed for viral load by Taqman Real Time PCR and genotype analysis was carried out by nested PCR using primers of core region to diagnose genotypes 1(1a/1b), 2, 3 and 4. RESULTS 67 patients were newly diagnosed with HCV infection during our study period were included in the study. Males were predominant 59.7%; most prevalent age group was 40-70 years (85%). Genotype 3 was predominant, 29 (43.3%), followed by genotype 1 in 28(41.8) and genotype 4 in 10 (14.9%) patients. High prevalence of genotype 3 was seen among female patients in contrary to genotype 4 in males. Among genotype 3, half of the patients had high viral load and in genotype 1b almost 60% had low viral load. But other genotypes 1a & 4 had high viral load in 3/4 of the patients. CONCLUSION Genotype 3 and 1 were almost equally distributed among our study population. Both genotype 1 and 4 showed increasing trend of prevalence. Our study emphasises that HCV genotyping in combination with quantitative evaluation of HCV RNA will be beneficial in selecting the duration of interferon treatment.
Authors and Affiliations
Raja Sundaramurthy, Vithiya Ganesan, Ramesh Arunagiri, Rajendran Thiruvannamalai, Geni Veerathevar German Soundaram, Brindha Vetri Nallathambi, Jhansi Charles
Keywords
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- EP ID EP553025
- DOI 10.14260/jemds/2019/10
- Views 93
- Downloads 0
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