Gram positive organisms isolated from neonatal septicaemia
Journal Title: Indian Journal of Microbiology Research - Year 2017, Vol 4, Issue 1
Abstract
Introduction: Neonatal septicaemia constitutes an important cause of morbidity, mortality and fatality amongst Indian neonates. A remarkable feature of the clinical manifestation of neonatal septicaemia is non-specificity of symptoms, which creates difficulty in diagnosis of infection in the early stage. Materials & Methods: 1-10 ml of blood collected and inoculated 10ml of Brain-Heart infusion broth. Sub culture on Blood agar, chocolate agar after 24 hrs with sterile inoculation loop (4mm) again after 48 hrs subculture on same culture plates and every day for five days. The organisms were identified by using different characteristic tests like, colony morphology, Gram’s stain, coagulase tests (both slide and tube) along with serological test procalcitonin test. Results: Neonatal septicaemia was seen in 82 cases, in that 53 cases were males and 29 cases were females. 45 cases (male) were isolated in early sepsis and 07 cases (females) were isolated in late sepsis. Procalcitonin test positive were in 67 cases in which 56 cases were positive for both blood culture and Procalcitonin test, 11 cases were positive to Procalcitonin test only and negative for blood culture. The organisms isolated in this study were Gram positive bacteria like Staphylococcus aureus, Coagulase negative Staphylococci (CONS) and few resistance strains of Staph. aureus like MRSA. Conclusion: A positive blood culture and PCT test are the only definitive and full proof methods of confirming the septicaemia. Hence, we conclude that in our study the best combination of tests in diagnosis for confirming neonatal septicaemia shows a high degree of sensitivity, specificity and positive predictive accuracy.
Authors and Affiliations
Nawaz Umar, S. Mohammed Shahid, Bilal Ahmad Mir
Keywords
Related Articles
- EP ID EP294010
- DOI 10.18231/2394-5478 .2017.0020
- Views 114
- Downloads 0
Aerobic Bacteriological Profile from Wound Site Infections in Road Traffic Accident (RTA) Patients
Background: Infections of musculoskeletal injuries due to road traffic accidents, especially open fractures, continue to pose a challenge. There is limited information on the early bacterial contaminants of these wounds...
Seropositivity to anti-thyroid peroxidase and anti-thyroglobulin autoantibodies in hypo and hyper-thyroidism: Diagnostic and epidemiological significance
Background: Autoantibodies to thyroid antigens in general, and thyroid peroxidase in particular, are considered as the hallmark of autoimmune thyroid dysfunctions. While a pro-inflammatory response is considered as predo...
Biosurfactant: Types, Detection Methods, Importance and Applications
There are many insoluble substrates that remain unavailable for macro and micro organisms. A microorganism synthesizes surface active agents to utilize these substrates that are collectively called as biosurfactants. Bio...
A study on carbapenemase producing Acinetobacter sp., and identification of OXA-51 gene in isolates from patients attending a tertiary care hospital
Introduction Acinetobacter sp are Gramnegative nonfermenters that causes nosocomial infections Multi drug resistance has been evolving very rapidly in Acinetobacter sp with also a dramatic increase in carbapenem resistan...
Clinico-microbial profile of Otomycosis in discharging otitis media in tertiary care hospital
Introduction: Discharging otitis media with superadded fungal infection is now become major otolarynological problems in India commonly seen in children and also in adults. The incidence has been increased in proportion...