Hepatitis C Virus Genetic Diversity and Drug Mutational Analysis in Cameroon: 1992 to 2013
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 1
Abstract
HCV infection is endemic in Cameroon. A Direct acting agent (DAAs), sofosbuvir that target the HCV non-structural 5B RNA-dependent RNA polymerase (NS5B RdRp) has recently been incorporated into the standard of care in Cameroon but no drug resistance study to this DAA has been reported in Cameroon. From the laboratory, 157 sequences were obtained and 252 downloaded from the Los Alamos HCV Sequence Database, of which 45 were Core (C), 112 were envelope (E1) and 252 were NS5B sequences were characterized by phylogeny. Drug resistance pattern in the NS5B gene was analyzed using the Geno2Pheno HCV 0.92 tool. Genotypes 1, 2 and 4 were identified. Several drug resistance-associated mutations in the NS5B gene that confer susceptibility (S282T (0.4%), and M414T (0.4%), I434M (2.8%), M414L (6.0%), C289L (13.5%) that confer possible resistance as well as (F415Y (41.3%) and V179A (4.0%) of unknown effect on sofosbuvir (SOF) were identified. There is a low level resistance rate to SOF in Cameroon.
Authors and Affiliations
Judith Ndongo Torimiro, Laure Arlette Tchapda, Maurice Boda, Jude Saber Bimela, Dale Netski, Désire Takou, Donald Scot Burke, Ubald Tamoufe, Eitel Mpoudi-Ngole, Henry Namme Luma, Oudou Njoya, Wilfred Fon Mbacham, Nathan Wolfe, Stuart Ray
Keywords
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- EP ID EP350929
- DOI 10.9734/BMRJ/2016/28823
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