Herpesviral and “Red Complex” Bacterial Analysis of Acute Apical Abscesses
Journal Title: Journal of Dental Problems and Solutions - Year 2015, Vol 2, Issue 3
Abstract
Introduction: To investigate the possible association between presence of Epstein–Barr virus, human cytomegalovirus and three endodontic bacterial pathogens (Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia) “red complex” in samples from patients with acute apical abscesses using real-time PCR. Methods: Periapical samples were collected from 18 acute apical abscess specimens and 6 control samples. The apical area of each study tooth was examined in periapical radiographs obtained using a long cone paralleling technique. The study included only single-rooted teeth that had carious lesions, necrotic pulps, and radiographic evidence of periradicular bone loss. No apparent communication from the abscess to the oral cavity or skin surface was observed. DNA extracts from purulent exudate aspirates of acute apical abscess and six control samples were evaluated for the presence of microbial and viral loads using real-time polymerase chain reaction (PCR) following the kit protocols recommended by the manufacturers. The chi squared test was used for statistical analysis. Results: HCMV, EBV, T. denticola, P. gingivalis, and T. forsythia were detected in 16.7 %, 5.6 %, 44.4 %, 27.7 %, and 22.2 % of the samples, respectively. As for the healthy pulps used as noninflamed controls, no control specimens contained bacterial or viral DNA. The pair HCMV/ T. denticola and HCMV/ T. forsythia was detected in one case. The association between HCMV, T denticola and T. forsythia was not statistically significant. Conclusion: Within the limitations of the present study, our PCR based findings revealed that in “red complex” bacteria, T. denticola and T. forsythia tended to occur in co-infection with HCMV.
Authors and Affiliations
Selcuk M Ozbek, Ahmet Ozbek, Tayfur Demiray
Keywords
Related Articles
- EP ID EP206293
- DOI 10.17352/2394-8418.000019
- Views 119
- Downloads 0
Proinflammatory Cytokines and Periodontal Disease
Numerous biological procedures are strictly controlled by cellcell interactions, which are categorized into two forms: cognate (adhesive) interactions, attained by mutual recognition between membrane-bound cell-surface m...
General-leutnant Dr Karl Mauss (1898-1959)
After it has invaded Poland, Germany will have to carry on paying the bills for Hitler’s expansion projects. Thus, thousands of men will die on the battle fi elds. Many more will come back crippled. A lot of men abuse th...
International Dental Aid and WWII
During the liberation of the camps, a great majority of the detainees died after eating abundantly within a short period of time. A thousand men died because the Americans and the Englishmen were totally bewildered and o...
The procedure of Anteroposterior Tooth Contact Adjustment (APTCA) in Orthodontic patients
Orthodontic relapse is the major concern in both adolescent and adult orthodontic treatment. The causes of orthodontic relapse are reported to be of various origins. Functional diagnostic procedures allow to report the p...
Neonatal Teeth and Riga-Fede Disease
A 3 week-old male presented with ulceration on the ventral surface of the tongue. His parents informed that the patient had poor nutrients intake due to pain caused by tongue ulcer.