HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF MEFENAMIC ACID IN HUMAN PLASMA USING UV VIS DETECTOR
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objective: Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). This drug provides analgesic and antipyretic (fever reducing effect) and higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma.Methods: Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 (150 mm x 4.6 mm, i. d 3.5 µm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored at 280 nm using variable-wavelength detector.Results: The recovery was found 83% for MA. The method was validated according to the Centre for Drug Evaluation and Research (CDER) guidelines. Calibration plot was linear within the range from 250 to 5000ng ml-1 with the coefficient of determination (r2) of ≥ 0.99. The quality control samples of mefenamic acid which was termed as low (L), medium (M) and high (H) were analysed to get the precision and accuracy. The accuracy for intra-day for L, M and H was 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient variance (CV) for intra-day precision for L, M and H was found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study.Conclusion: The results were in compliance with CDER guideline.Â
Authors and Affiliations
Abm Helaluddin, Huda Jamilah Mohamad, Mohamed Alaama, Noorsyafawati Amiruddin
Keywords
Related Articles
- EP ID EP579281
- DOI -
- Views 107
- Downloads 0
IN VITRO TOTAL PHENOLICS, FLAVONOIDS CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITES OF VARIOUS SOLVENT EXTRACTS FROM THE MEDICINAL PLANT PHYSALIS MINIMA LINN
Objective: To estimate the in vitro total phenolics, flavonoids contents, antioxidant and antimicrobial activities of various solvent extracts from the medicinal plant Physalis minima Linn.Methods: The crude bioactive we...
ASSESSING THE IMPACT ON PEFR AMONG SMOKERS AND PASSIVE SMOKERS–A COMPARATIVE STUDY
Objective: The purpose of this study was to monitor the intensity and difference in Peak Expiratory Flow Rate (PEFR) between smokers and passive smokers. Methods: A total of 1000 participants were enrolled in two groups...
TOXICITY EVALUATION OF HYDRO-ALCOHOLIC EXTRACT OF PORTULACA OLERACEA (WHOLE PLANT) IN SWISS ALBINO MICE
Objective: To evaluate the toxicity (acute and sub-acute) of hydro-alcoholic extract of Portulaca oleracea (whole plant) in Swiss albino mice.Methods: Acute oral toxicity study was carried out as per OECD 423 guidelines....
ASSESSMENT OF PHYSICIANS' RESPONSE TO TEXT ALERTS IN PATIENTS WITH REDUCED RENAL FUNCTION BY USING A TEXT MESSAGE ALERTING SYSTEM
Introduction: Adverse drug events are mostly dose dependent and preventable. About 50% of these adverse effects are due to inappropriate dosing especially in patients with renal failure.Objective: We aimed to determine t...
MICROWAVE ASSISTED EXTRACTION; PHYTOCHEMICAL EVALUATION OF MALAYSIAN PALM OIL TRUNK EPIPHYTES FERNS
Objective: The objective of this research was to evaluate the, total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity, cytotoxicity and functional group of epiphytes ferns grows on the Malays...