High Resolution TEM Characterisation of Hydrogen Peroxide Treated Tooth Structures
Journal Title: International Journal of Dentistry and Oral Science (IJDOS) - Year 2015, Vol 0, Issue 1
Abstract
Objective: Previous studies reported that bleaching agents are capable of altering the outer enamel or exposed dentine surfaces. In this study, transmission electron microscopy (TEM) characterisation in combination with energy dispersive x-ray spectroscopy (EDS) elemental mapping was performed to investigate the effect of hydrogen peroxide (HP) on the sub-surface of tooth structures. Materials and Methods: Bovine incisors (n=6) were assigned to three groups for treatments: 30% H2O2 pH 3, 30% H2O2 pH 7 and 1M sodium hydroxide (NaOH) for 16 hours. Samples were exposed to direct treatment with agents to allow easy access of the agents and uniform treatment across the entire sample sub-surfaces. Specimens were immersed in Karnovsky’s fixative for three days, embedded in epoxy and polished. Using focussed ion beam (FIB), samples were milled ~50-100 μm below the treated polished surface to feature the ultra-fine structures for TEM analysis. Results: Enamel rods, inter-rods and crystalline structures within rods were discerned for sound and H2O2 treated enamel. No structural difference in the mineral dense peritubular region or fibrous protein rich inter-tubular dentine was observed after acidic or neutral H2O2 treatment. A complete loss in the structural integrity of enamel rod was observed after NaOH treatment for 16 hours without having any impact on dentine structure. Conclusions: TEM analysis produced high quality sub-surface images of tooth structures and revealed no deleterious effect on the structural integrity of the sub-surface enamel or dentine after direct bleaching with hydrogen peroxide.
Authors and Affiliations
Veena Mohan
Keywords
Related Articles
- EP ID EP201207
- DOI 10.19070/2377-8075-SI07001
- Views 175
- Downloads 0
Diagnostic Potential of Mandibular Second Premolar and its Relation to Physiological and Skeletal Maturity in Girls
Aims and objectives: 1). To estimate the association between mandibular second premolar calcification stages and cervical vertebrae maturation 2). To estimate relationship of dental maturation and sexual maturation in fe...
The Detection of Serum and Urine Levels of Alpha 2-Macroglobulin in Nasopharyngeal Carcinoma Patients Receiving Late Period Radiotherapy and its Significance
Objective: To detect the dynamic changes of the serum and urine alpha2-macroglobulin (α2M) in patients with nasopharyngeal carcinoma after receiving different doses of ionizing radiation and to provide further basis of α...
Effect of Arch Form and Water Sorption on the Palatal Base Adaptation of ProBase Hot Versus the Conventional Heat Cured Acrylic Resin
Background: The greater the curvature of the tissue at the posterior palatal region, the greater the distortion of the denture base. Purpose: This study was carried out to evaluate the effect of the arch form and water s...
High Resolution TEM Characterisation of Hydrogen Peroxide Treated Tooth Structures
Objective: Previous studies reported that bleaching agents are capable of altering the outer enamel or exposed dentine surfaces. In this study, transmission electron microscopy (TEM) characterisation in combination with...
Pfeiffer Syndrome Type 1: A Case Report
Pfeiffer syndrome is a rare syndrome which is defined by Pfeiffer in 1964 is autozomal dominant and characterized with variable severity of brachycephalic skull, regressed midface, expansion in hands and feet, broad thum...