Histochemical Effects of “Verita WG” on Glycogen and Lipid Storage in Common Carp ([i]Cyprinus carpi[/i]o L.) Liver
Journal Title: Ecologia Balkanica - Year 2013, Vol 5, Issue 2
Abstract
We aimed in the present work is to study the effects of fosetyl-Al and fenamidone based fungicide (“Verita WG”) on glycogen storage and expression of lipid droplets in common carp ([i]Cyprinus carpio,[/i] L.) liver. Concentrations of the test chemical were 30 mg/L, 38 mg/L and 50 mg/L under laboratory conditions. We used PAS-reaction for detection of glycogen storage and Sudan III staining for detection of lipid droplets in common carp hepatocytes. Hence, we found that the amount of glycogen and the fat storage in the liver increased proportionally with the increased fungicide concentrations. We also found conglomerates of accumulated glycogen in certain hepatocytes at all used concentrations. Overall, the results demonstrated enhanced glyconeogenesis and fat accumulation in the common carp liver, exposed to the test chemical.
Authors and Affiliations
Elenka Georgieva, Pepa Atanasova, Iliana Velcheva, Stela Stoyanova, Vesela Yancheva
Keywords
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