HISTOMORPHOLOGICAL RESPONSE OF SULFORAPHANE AND RIBOCEINE ON ANNULAR PUNCTURE- INDUCED MODEL OF RABBITS INTERVERTEBRAL DISC DEGENERATION

Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 11

Abstract

Background: Intervertebral disc degeneration is one of the leading causes of Low back pain that is regarded as one of the most frequent neurological ailments. Objective: To determine the regenerative potential of sulforaphane and riboceine supplements in a rabbit model in annular punctured intervertebral disc degeneration. Methods: Forty New Zealand white rabbits (3 to 3.5 kg each) underwent annular puncture of the L2-L3, L3-L4, and L4-L5 discs. Rabbits were sacrificed at 2, 4 and 8 after puncture. For a longitudinal study to assess changes in disc height over time, serial X-rays were performed at 0, 4 and 8 weeks for the rabbits. Upon sacrifice, the whole spinal column and discs were extracted and analyzed with real time reverse transcriptase-polymerase chain reaction, and histological staining. Results: The radiology assessment revealeda significant progressive decrease in disc height which was reversed by the administration of sulforaphane and riboceine. Significant disc space narrowing compared to preoperative disc height was observed during the time period (p<0.05). The histological grade, collagen type 1 and 2, aggrecan, and matrix metalloprotease-13 mRNA expression and Hematoxylin and Eosin were indicative of degeneration, supporting the results of the radiology. Conclusion: Administration of sulforaphane and riboceine in this study revealed the regenerative potential of nutritional supplement in intervertebral disc. The annular puncture model can be used to study and validate the therapeutic potential of drugs and biological materials in the treatments of degenerative disc disease.

Authors and Affiliations

Adelakun S. A.

Keywords

Related Articles

MORPHOMETRIC EVALUATION OF THE EXTERNAL EAR IN RELATION TO ATTACHMENT PATTERN: A STUDY OF ADULT NIGERIANS RESIDENT IN THE SOUTHERN REGION

There are two configurations of earlobe in humans; attached and unattached earlobe. This study was done to evaluate the morphology of the external ear in relation to attachment pattern and to determine the association be...

EARLY DIAGNOSIS AND TREATMENT OF ISOLATED MAXILLARY SINUS DISEASES

The maxillary sinus or antrum of Highmore is a hollow space between the craniofacial skeleton which is lie in the maxillary bone. It is a pyramid shape cavity filled with air in adjacent location to the nasal and oral ca...

KAWASAKI DISEASE IN CHILDREN PRESENTED TO MAKASSED GENERAL HOSPITAL: A SEVENTEEN YEAR REVIEW

Background: Kawasaki disease is an acute febrile illness of early childhood, with about 80% of cases occurring between 6 months and 5 years. It is characterized by fever lasting at least five days and a constellation of...

SURVEILLANCE ON COMMUNICABLE DISEASE –INFLUENZA

For better control of communicable diseases, National Communicable Disease Surveillance Systems must be strengthened. Communicable diseases have always been as one of the main threats for human health. Influenza, or flu,...

SUBSTANCE USE AND ITS CONTRIBUTING FACTORS AMONG MALE AND FEMALE IN SELECTED VDC OF DANG DISTRICT, NEPAL: A COMPARATIVE STUDY

Background: Substances are things like alcohol, tobacco, smoking which are used by people as the mean of entertainment, coping from stress and some cultural practice of specific groups. Substance use poses major politica...

Download PDF file
  • EP ID EP630358
  • DOI -
  • Views 169
  • Downloads 0

How To Cite

Adelakun S. A. (2017). HISTOMORPHOLOGICAL RESPONSE OF SULFORAPHANE AND RIBOCEINE ON ANNULAR PUNCTURE- INDUCED MODEL OF RABBITS INTERVERTEBRAL DISC DEGENERATION. European Journal of Biomedical and Pharmaceutical Sciences, 4(11), 1-9. https://www.europub.co.uk/articles/-A-630358