HISTOMORPHOLOGICAL RESPONSE OF SULFORAPHANE AND RIBOCEINE ON ANNULAR PUNCTURE- INDUCED MODEL OF RABBITS INTERVERTEBRAL DISC DEGENERATION
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 11
Abstract
Background: Intervertebral disc degeneration is one of the leading causes of Low back pain that is regarded as one of the most frequent neurological ailments. Objective: To determine the regenerative potential of sulforaphane and riboceine supplements in a rabbit model in annular punctured intervertebral disc degeneration. Methods: Forty New Zealand white rabbits (3 to 3.5 kg each) underwent annular puncture of the L2-L3, L3-L4, and L4-L5 discs. Rabbits were sacrificed at 2, 4 and 8 after puncture. For a longitudinal study to assess changes in disc height over time, serial X-rays were performed at 0, 4 and 8 weeks for the rabbits. Upon sacrifice, the whole spinal column and discs were extracted and analyzed with real time reverse transcriptase-polymerase chain reaction, and histological staining. Results: The radiology assessment revealeda significant progressive decrease in disc height which was reversed by the administration of sulforaphane and riboceine. Significant disc space narrowing compared to preoperative disc height was observed during the time period (p<0.05). The histological grade, collagen type 1 and 2, aggrecan, and matrix metalloprotease-13 mRNA expression and Hematoxylin and Eosin were indicative of degeneration, supporting the results of the radiology. Conclusion: Administration of sulforaphane and riboceine in this study revealed the regenerative potential of nutritional supplement in intervertebral disc. The annular puncture model can be used to study and validate the therapeutic potential of drugs and biological materials in the treatments of degenerative disc disease.
Authors and Affiliations
Adelakun S. A.
Keywords
Related Articles
- EP ID EP630358
- DOI -
- Views 168
- Downloads 0
DIGIT RATIO OF PRISON INMATES WITHIN SOUTH-SOUTH NIGERIA
Digit ratio is a putative biomarker for the concentration of testosterone in utero. The present study was aimed at documenting the values of the digit ratio of the prison inmates within the South-South region of Nigeria....
EVALUATION OF UTERINE MALFORMATIONS OF PATIENTS ATTENDING MADONNA UNIVERSITY TEACHING HOSPITAL USING ULTRASOUND
Ultrasound is a common diagnostic tool used for the assessment of uterine morphology over several decades.This research is to determine the various uterine abnormalities observed in patients who underwent ultrascan in Ma...
ASSESSMENT OF VITAMIN D LEVELS IN SUDANESE PATIENTS WITH SICKLE CELL DISEASE AND ITS IMPACT ON SICKLE CELLS COMPLICATIONS-KHATOUM-2017
Sickle cell disease SCD is a common reason urging patients of African descent to seek emergency medical care.[1] This study was done in Khartoum state in a period between March 2015 until July 2015. This study was done t...
MULTIVARIATE CHEMOMETRIC MODELS APPLIED FOR SIMULTANEOUS DETERMINATION OF ROSUVASTATIN CALCIUM AND TELMISARTAN IN PURE FORM AND IN PHARMACEUTICAL PREPARATION; A COMPARATIVE STUDY
Objective: Experimental design of different synthetic mixtures of rosuvastatin calcium and telmisartan in different ratios were constructed. The zero-order absorption spectra of these prepared mixtures have been recorded...
POLYMORPHISM IN THE GLUTHATHIONE S-TRANSFERASE THETA AND MU GENES AND SUSCEPTIBILITY TO LYMPHOID LEUKEMIA IN SUDANESE PATIENTS
Background: Glutathione S-transferase (GST) are a family of cytosolic enzymes known to catalyze the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products...