Histopathological and Immunohistochemical Evaluation of Follicles of Asymptomatic Impacted Third Molars and their Pathological Risk Assessment
Journal Title: Journal of Advanced Medical and Dental Sciences Research - Year 2018, Vol 6, Issue 2
Abstract
Introduction- Dental follicle and dentigerous cyst share common histopathological features. The present study was undertaken to evaluate and correlate the histopathological findings of the dental follicles with the dentigerous cyst and to compare the immunohistochemical expression of molecular markers bcl-2 and Ki-67 in dental follicles compared and dentigerous cyst. Materials & Methods- The study was carried out on 50 dental follicles obtained after surgical removal of impacted third molars and 10 archival tissue blocks of histopathologically confirmed cases of dentigerous cyst which were used as control. All the cases were examined histopathologically using routine haematoxylin and eosin staining (H & E) and special stains (PAS) for determining pathological changes in epithelial lining and connective tissue. Results- 14 out of 40 cases (35%) of dental follicle and 6 out of 10 cases (60%) of dentigerous cysts showed reduced enamel epithelial lining. 22 out of 40 cases of dental follicles and 4 out of 10 cases of dentigerous cysts showed squamous metaplasia. 8 out of 40 cases of dental follicles and 4 out of 10 cases of dentigerous cysts showed mucous prosoplasia. Squamous metaplasia was evident in 5 out of 14 cases of reduced enamel epithelium. Maximum number of cases showing dense collagenous type of connective tissue was present in generalized proliferating type of epithelium. 75% dental follicles had dense collagen connective tissue and 60% of dentigerous cysts had dental papillae type of connective tissue and plump shaped fibroblasts. The mean value (labeling index) of Ki-67 in positive cases of dental follicles (4.20±4.043). Strong staining was seen in 2 cases of reduced enamel epithelium, 10 cases of generalized proliferating epithelium. Conclusion- A simultaneous need to conduct studies based on clinical, histopathological immune-histochemical and genetic factors to elucidate the actual inherent potential of the dental follicles to develop pathologies.
Authors and Affiliations
Neha Pruthi, Devi Charan Shetty, Raghu Dhanapal
Keywords
Related Articles
- EP ID EP503199
- DOI -
- Views 118
- Downloads 0
Estimation of Stature from Femur Length- A Forensic Study
Background: Stature estimation forms a major domain of medico-legal investigations used in the identification of unknown fragmentary and mutilated remains. The present study was conducted to assess the stature of person...
IMPACT OF ORAL HEALTH RELATED QUALITY OF LIFE OF PERIODONTALLY COMPROMISED PATIENTS
Background: Oral disorders cause considerable physical pain and suffering, impairment of function and reduced quality of life. Periodontal disease may compromise functional aspects of the stomatognathic system, such as m...
Burkholderia pseudomallei Infection- A Review
Clinical patterns of infection caused by Burkholderia pseudomallei are generally consistent with those from South and Southeast Asia interms of common primary presentations with diabetes as a major risk factor. Early dia...
AN APPROACH TO EVALUATE THE ORAL PEDIATRIC BIOPSIES- A RETROSPECTIVE STUDY
Aim and Objectives: The aim of the study was to evaluate retrospectively, the pediatric oral biopsies received in last 1years from the institute. To evaluate the total number of pediatric oral biopsies obtained below th...
Assessment of Oral Lesions in Patients with Anxiety and Depression in Jammu Population
Background: A psychosocial disorder is a mental illness caused or influenced by life experiences, as well as maladjusted cognitive and behavioural processes. Literature quotes numerous studies highlighting prevalence of...