HIV-1 subtypes and drug-resistance mutation sites in HIV/AIDS patients with antiretroviral-therapy failure
Journal Title: Shanghai Journal of Preventive Medicine - Year 2023, Vol 35, Issue 3
Abstract
ObjectiveTo analyze the characteristics of HIV-1 subtypes and drug-resistance mutation sites among HIV-infected patients who received high-efficiency antiretroviral therapy but failed.MethodsA total of 130 plasma samples were collected from the patients who received antiviral treatment for 6 months in Taizhou City of Zhejiang Province in 2021 but failed the treatment and the viral load was â¥1 000 copies·mL-1. Nucleic acid in the samples was extractedï¼ and the pol gene was amplified by nested reverse transcription PCR. After next-generation sequencingï¼ online tools were used to compare and analyze the subtypes and drug-resistant mutation sites.ResultsA total of 110 samples were successfully sequenced. The main HIV-1 subtype was CRF01_AEï¼ accounting for 42.72% ï¼47 casesï¼ï¼ followed by CRF07_BCï¼ 35.45% ï¼39 casesï¼ï¼ CRF08_BCï¼ 10.00% ï¼11 casesï¼ï¼ CRF85_BC ï¼ 8.18% ï¼9ï¼ï¼ and a small number of B subtypeï¼ 1.81% ï¼2 casesï¼ and C subtypeï¼ 1.81% ï¼2 casesï¼. The online tool comparison showed that there were 67 cases with mutations of drug-resistance sites and 61 cases with drug-resistance. The mutation sites were mainly M184Vï¼ K103Nï¼ K65R and V181Cï¼ and the mutation rates were 20.00% ï¼22 casesï¼ï¼ 10.91% ï¼12 casesï¼ï¼ 8.18% ï¼9 casesï¼ and 8.18% ï¼9 casesï¼ï¼ respectively. These mutation sites caused different degrees of resistance to nucleoside reverse transcriptase inhibitors ï¼NRTIï¼ï¼ non- nucleoside reverse transcriptase inhibitors ï¼NNRTIï¼ and protease inhibitors ï¼PIï¼ï¼ including 45 cases of NRTIï¼ 61 cases of NNRTI and 2 cases of PI resistance.ConclusionThe HIV infected people who fail the treatment in Taizhou are mainly with the subtypes CRF01_AE and CRF07_BC. The rate of drug-resistance mutation is at a moderate levelï¼ mainly due to the mutation of NRTI and NNRTI drug-resistance sitesï¼ and a small number of PI drug-resistance sites. Thereforeï¼ the antiviral treatment plan for HIV infected people should be reasonably adjustedï¼ and the detection of drug-resistance mutation sites should be strengthened to avoid the generation of transmissible drug-resistance strains.
Authors and Affiliations
LIN Junxiao,WANG Hongzhu,LI Congcong,SHENG Ying,LI Guixia,
Keywords
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