HPLC METHOD DEVELOPMENT AND VALIDATION: SIMULTANEOUS DETERMINATION OF ACTIVE INGREDIENTS IN COUGH AND COLD PHARMACEUTICALS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 10
Abstract
Objective: This study aimed to develope a simple reversed-phase high performance chromatographic method for simultaneous determination of pseudoephdrine HCI, pheniramine maleate, acetaminophen, guaifenisin, pyrilamine maleate, chlorpheniramine maleate, triprolidine HCI, dextromethorphan HBr, diphenhydramine HCI in cough and cold pharmaceuticals.Methods: The separation of these compounds was achieved within 37.9 min on a Nucleodur gravity C18 column (250 x 4.0 mm, 5μm). The chromatographic separation of these compounds performed in a single run by using isocratic mobile phase consisting of methanol:buffer mixture (38:62, v/v) at room temperature, with flow rate of 0.75 mL.min-1.An ultraviolet absorption at 210 nm was monitored. 2,4,6-trimethoxybenzaldehyde was used as an internal standard (ISTD). The selectivity, linearity of calibration, accuracy, intraday and interday precision and forced degradation studies were examined as parts of the method validation.Results: The concentration–response relationship was linear over a concentration range of 0.2-250 µg.mL-1 for acetaminophen, 0.5–250 µg.mL-1 for pseudoephdrine HCI and pheniramine maleate, 1–250 µg.mL-1 for guaifenisin, 2.5-250 µg.mL-1 for chlorpheniramine maleate and triprolidine HCI, 5-250 µg.mL-1 for pyrilamine maleate and diphenhydramine HCI, 10-20 µg.mL-1 for dextromethorphan HBr with correlation coefficients better than 0.9993. The relative standard deviations of the intraday and interday were all less than 4%. Conclusion: The proposed liquid chromatographic method was successfully applied for the routine analysis of these compounds in different cough and cold pharmaceutical preparations such as syrups and tablets.Â
Authors and Affiliations
Hatice Çağlar, Ebru Büyüktuncel
Keywords
Related Articles
- EP ID EP579477
- DOI -
- Views 85
- Downloads 0
EXPERIMENTAL EVIDENCES FOR THE PROMISING HERAPEUTIC ROLE OF VITIS VINIFERA SEED EXTRACT AGAINST NONALCOHOLIC STEATOHEPATITIS
Objective: The present study was planned to investigate the possible therapeutic effect of methanolic extract of Vitis vinifera seeds on high fat diet-induced nonalcoholic steatohepatitis (NASH) in forty adult female Wis...
IN SILICO ANALYSIS OF INHIBITOR AND SUBSTRATE BINDING SITE OF SERRAPEPTIDASE FROM SERRATIA MARCESCENS MTCC 8708
Objective: Serrapeptidase is a therapeutic enzyme broadly used as an anti-inflammatory drug to treat inflammatory diseases like arthritis, bronchitis, fibrocystic breast disease and sinusitis. The objective of present st...
PRELIMINARY PRELIMINARY STUDY ON BLOOD PROFILE OF BLOOD PRESSURE AND VEGETABLE CONSUMPTION AMONG WOMEN IN THE ERA OF GERMAS IN CICANIR AND JATIPAMOR VILLAGES, MAJALENGKA REGENCY, WEST JAVA PROVINCE
Objective: To discover the profile of hypertension (HT) disease and consumption habit of vegetables among women in Cicanir and Jatipamor villages related to the program of Gerakan Masyarakat Hidup Sehat (GERMAS)/Healthy...
NEPHROPROTECTIVE, NEPHROCURATIVE ACTIVITY OF MIMOSA PUDICA ROOT AGAINST GENTAMICIN INDUCED NEPHROTOXICITY
Objective: To study nephroprotective effect of ethanolic extract of Mimosa pudica root against Gentamicin induced nephrotoxicity in rats. Methods: After the treatment scheduled period of 21 days, the extent of nephrotox...
TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF SILYBUM MARIANUM CULTURES GROWN ON DIFFERENT GROWTH REGULATORS
Objective: The present study was designed to study the effect of supplementing Silybum marianum cultures with different combinations and concentrations of growth regulators on the total phenolic content and the antioxida...