HPLC/MS/MS Study of Phenolic Compounds of Leucaena leucocephala Legumes Monitored with Their in vitro Antihyperglycemic Activity
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 17, Issue 4
Abstract
High pressure liquid chromatography coupled with mass spectrometry (HPLC/MS/MS) is considered as one of most the sensitive modern techniques used for determination of phenolic compounds, hence in this research it was utilized for characterization of a variety of phenolic compounds in Leucaena leucocephala legumes growing in Egypt. HPLC gradient elution analysis using water and acetonitrile, both containing 0.1% formic acid was carried out for standard solutions of the available phenolic and flavonoid compounds as well as the investigated fraction in the negative ion mode using MS/MS product ion scans where the deprotonated molecules [M‒H]- were observed for all the studied compounds; for flavonol and flavone glycosides, the spectra exhibit both the deprotonated molecule [M‒H]‒ of the glycoside and the ion corresponding to the deprotonated aglycone [A‒H]–, the latter ion is formed by loss of the sugar residue from the glycosides; different fragmentation pattern was observed for flavone-C-glycosides which involved additional fragmentation in the sugar part while for methyl ether aglycones, formation of [M‒CH3]‒ • was observed. The gained results revealed the elution of eleven phenolic compounds from the ethyl acetate fraction, namely; isovanillin, gallic acid, caffeic acid, apigenin-8-C-glucoside (vitexin), quercetin 3-O-galactoside (hyperoside), luteolin-7-O-glucoside, quercetin, isorhamnetin, luteolin 6-methyl ether (neptein), kaempferol 3,7-dimethyl ether and 5,7,3',4'-dihydroquercetin tetramethyl ether (taxifolin 5,7,3',4'- tetramethyl ether) successively. The methanol extract and different fractions of Leucaena leucocephala legumes were subjected to in vitro evaluation of their α-glucosidase inhibitory activity at different dose levels compared to the reference standard drug “Acarbose”, the gained results revealed that methanol extract and ethyl acetate fraction exhibited significant percent inhibitory activity at dose level of 2.5 mgml-1 (71.55±0.009%, IC50 0.210±0.003 mg ml-1 & 65.44±0.005%, IC50 0.270±0.042 mg ml-1) respectively compared to Acarbose (55.45±0.051%, IC50 0.650±0.051 mg ml-1) which affords both as effective natural antihyperglycemic drugs.
Authors and Affiliations
Nevein M. Abdelhady, Gamil M. Abdallah
Keywords
Related Articles
- EP ID EP349615
- DOI 10.9734/EJMP/2016/31403
- Views 114
- Downloads 0
Antiplasmodial Potential and Phytochemical Screening of Ten Plants Used as Antimalarial in Mali
Aim: This study was designed to determine in vitro antiplasmodial activities of extracts from ten Malian medicinal plants against P. falciparum strains. Place and Duration of Study: Collection of plant materials and bas...
Acaricidal Activities of Hyptis suaveolens and Ocimum sanctum Against African Dog Tick (Rhipicephalus sanguinneus)
Aim: To determine the acaricidal activities of some solvent extracts (chloroform, methanol and n-hexane) and crude extracts of Hyptis suaveolens and Ocimum sanctum against African dog tick (Rhipicephalus sanguinneus). S...
Anticancer Activity of Three Jamaican Macroalgae against Prostate, Pancreatic and Skin Cancers
Aims: Cancer is one of the leading chronic diseases that may lead to death. The search for new therapeutic, medicinal and nutraceutical compounds from folkloric plants including the marine flora are globally important ob...
Preliminary Phytochemical, Acute Oral Toxicity and Anticonvulsant Activity of the Seed Extract of Brassica juncea
Aims: The aim of this research is to investigate the preliminary phytochemical properties, acute oral toxicity and anticonvulsant activity of the seed extract of Brassica juncea (B. juncea). Study Design: Qualitative an...
Toxicity and Pharmacognostic Standards for Laxative Properties of Nigerian Cassia sieberiana and Senna obtusifolia Roots
Aims: Research for sourcing laxative crude drugs among Nigerian Cassia/Senna species has continued. The roots of Cassia sieberiana and Senna obtusifolia, reported as mild laxatives, were subjected to pharmacognostic and...