Human micro- and macrovessel-derived endothelial cells: a comparative study on the effects of adrenaline and a selective adenosine A(2)-type receptor agonist under normoxic and hypoxic conditions.
Journal Title: Pharmacological Reports - Year 2007, Vol 59, Issue 6
Abstract
Adrenaline is a highly effective stimulator of cyclic AMP (cAMP) production in microvascular endothelial cells (ECs) - HMEC-1, showing only a moderate activity in macrovascular ECs - HUVEC. In both EC preparations, adrenaline acts via beta-type receptors. Selective stimulation of adenosine A(2)-type receptors resulted in comparable increases in cAMP formation in ECs lining micro- and macrovessels. Hypoxia largely suppressed the cAMP effects resulting from stimulation of both beta-adrenoceptors and adenosine A(2) type receptors in ECs of microvessels (HMEC-1). In contrast, hypoxia had only slight effect on these responses in ECs of macrovessels (HUVEC). The present data provide further evidence of functional differences between microvessel- and macrovessel-derived ECs.
Authors and Affiliations
Anna Wiktorowska-Owczarek, Magdalena Namiecinska, Aneta Balcerczyk, Jerzy Nowak
Keywords
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