Identification and Antimicrobial Susceptibility testing of Non Fermenting Gram Negative Bacteria by Vitek 2 in a Teaching Hospital in Mahbubnagar
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2018, Vol 7, Issue 10
Abstract
Non fermenting gram negative bacilli (NFGNB) are aerobic, non-spore forming bacteria. Although previously considered as contaminants, their pathogenic potential was proved by their frequent isolation from clinical samples and association with diseases. Aim: To identify NFGNB from various clinical samples and perform antimicrobial susceptibility testing (AST) by Vitek 2. Materials and methods: All the clinical samples received for routine bacterial culture in Department of Microbiology, SVS Medical College, from January 2017 to April 2018 were plated on agar plates and non-lactose fermenting colonies on MacConkey agar plates were further processed by Vitek 2. Results: A total of 126 NFGNB were isolated from various clinical samples and their AST was evaluated by Vitek 2. Pseudomonas aeruginosa (66.66%) and Acinetobacter baumanii (23.80%) were most common NFGNB isolated. High variability in antibiotic resistance pattern was noted in the present study. 47.61 % of NFGNB exhibited Multidrug resistance (MDR) and 3.37% Pseudomonas aeruginosa strains showed Pandrug resistance (PDR). Conclusion: Accurate and rapid identification and antimicrobial susceptibility testing of NFGNB by Vitek 2, help in early initiation of appropriate antimicrobial therapy and proper management of patients and will reduce emergence of MDR and PDR strains of NFGNB.
Authors and Affiliations
Srividya Yeruva, Vasantha Kabra
Keywords
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- EP ID EP582858
- DOI 10.20546/ijcmas.2018.710.024
- Views 111
- Downloads 0
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