Identification of B(A) subtype and analysis of bood transfusion strategiaes
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 8
Abstract
Objective To investigate the serology and genotype identification method of B (A) subtype patients. Methods Test tube method (serology) was used to confirm the clinically difficult ABO blood group samples of 3 patients with ABO blood group; ABO blood group was genotyped by real-time PCR, and the ABO gene exon 1-7 was sequenced to determine the genotype. Results The forward and reverse blood typing result of three patients was B (A) subtype all with ABO genotype B/O2 and c.640A> G mutation on B allele of exon 7, which meets the characteristics of ABO * BA.04 genotype. Conclusion The combination of serological and genetic testing could identify difficult blood types such as ABO subtypes accurately and ensure the safety of clinical blood use.
Authors and Affiliations
Chunxia LIU, Lu NIU, Cuicui CHAI, Han WANG, Hongliang ZHANG, 宏军 高
Keywords
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- EP ID EP738610
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.08.007
- Views 65
- Downloads 0
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