Immunohistochemical Expression of Toll-Like- Receptor-2 in Oral Epithelial Dysplasia Induced In Experimental Rats and Its Possible Relation to Mast Cell Count (An Animal Study)
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 6
Abstract
TLR-2 is a protein that is present in many cells, but its activation occurs due to microbial infection or genetic mutation. TLR-2 was significantly associated with the aggressive behavior of OSCC. Although the phenotypes of TLR-2 in different types of solid tumors have been extensively characterized, the expression and functional properties of TLR-2 in premalignant lesions remain to be determined. The infiltration of MCs has been shown to be an early and predictablecharacteristic of many pre-neoplastic cells, and may represent one of the earliest indications that a cell population has become committed to malignancy. This study was conducted to evaluated the expression of TLR-2 in OED and correlate it with MCC. A total of 48 adult male albino rats with an age range of 3 to 4 months and with a weight range of 100 to 200 gm were selected. The rats were randomly distributedinto two groups: control and experimental groups. Each group was further subdivided into two subgroups according to the time of sacrifice (after 6 and 9 weeks from the start of painting). Six rats were housed under the same conditions and did not receive any treatment. Forty two rats were anesthetized by ketamine 80-100 mg/kg intraperitonealy and xylazine 10-12.5 mg/kg (IP). The buccal mucosa was paintedwith a number 3 camel hairbrush. The rats had their buccal mucosa painted (topically) with DMBA and formaldehyde, 0.5% DMBA in acetone 3 days/week, and after 9 days 10% formaldehyde/water was used side by side with DMBA throughout the study period (6 and 9 weeks). Euthanasia of rats was done at 6 weeks (group A1andB1) and 9 weeks (group A2 and B2) interval from the start of painting The rats Euthanasia by an overdose of the anesthetic agents (1ml/100gm). The dissectedbuccal mucosa of rats were processed routinely to obtain 5μ thick sections. Thesesections were examined histologically by H&E stain and immunohistochemically byTLR-2, and TB stain, a special stain to detection the MCs. Moreover, the areapercentage of TLR-2 immunoexpression and the number of MCs in the differentintervals were measured by software Leica Qwin 500.The results showed a highly significant difference in both area percentage ofTLR-2 immunoexpression and mean MCC among the different experimental groups.After six weeks, the mean area percent of TLR-2 immunoexpression and themean number of MCs in low risk OED was significantly greater than that of controlgroup. Similarly, after nine weeks of the experiment, the mean area percent of TLR-2immunoexpression and the mean number of MCs in high risk OED was significantlygreater than that of control group. In addition there was a strong significantcorrelation between area percentage of TLR-2 and number of MCs among the studiedgroups.In conclusion, the immunoexpression of TLR-2 and MCC differ from low riskto high risk OED compared to normal buccal mucosa. TLR-2 and MCC could beused as early markers for malignant transformation
Authors and Affiliations
Dr. Omar. A. Rageh B. D. S, Prof. Dr. Sawsan Naguib Abdel Bary, Ass. Prof. Dr. Nermine Raouf Ameen
Keywords
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- EP ID EP375911
- DOI 10.9790/0853-1706045376.
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