Immunological and Molecular study of Toxoplasma gondii from aborted women in Diyala / Iraq
Journal Title: Scientific Journal of Medical Research - Year 2018, Vol 2, Issue 6
Abstract
Objective: To determine the infection rate of T. gondii in aborted women, and to investigate the relationship between toxoplasmosis and some of the sociodemographic factors. Methods: The study included one hundred women divided in two groups; group 1: 58 aborted women, group 2: 42 pregnant women with previous abortion and 50 women with close ages who had no abortion or pregnancy were considered a control group. All were attend to Al-Batool Maternity Teaching Hospital in Baqubah/ Diyala province, blood samples were collected during the period from 15thAugust 2016 till 15thDecember 2017. Results: This study showed that 44(44%) were positive by ELISA (IgG &IgM) which considered as a confirmed toxoplasmosis cases, 4 (4%) of them had IgM+, 38 (38%) had IgG+, and 2(2%) had both IgM+ and IgG+, whereas the control women were 0% for all types of antibodies. The highest results of these tests were observed in 25-34 and 35-44 years age in non-pregnant and pregnant women, The present study suggest that there is a significant relationship between the pregnant women with T. gondii and the time and number of abortion. Real Time Polymerase Chain Reaction test in blood of pregnant and non-pregnant women has advantages in detection of recent or active toxoplasmosis. 15/100 (15%) of aborted women were positive by RT-PCR for toxoplasmosis associated with low avidity IgG, 4 of abortive women with IgM+ were RT-PCR positive. In addition, 7 of abortive women with IgG+, 2 of abortive women with IgM+& IgG+ were RT-PCR positive. 2 cases of abortive women with IgM-& IgG- were RT-PCR positive. Conclusion: The development diagnostic methods using ELISA IgG and IgM followed by an IgG-avidity assay was a step forward. As well as, the IgG avidity and RT- PCR methods in women were the best method in detection the recent and reactive infection by Toxoplasma gondii.
Authors and Affiliations
Nariman Hassan Darweesh, Rawaa A. Hussein, Sawsan Talib Salman, Mohammed Jasim Shaker
Keywords
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