Impact of Cold Stress on Physiological, Hormonal and Immune Status in Male and Female Broad Breasted White Turkeys
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2013, Vol 9, Issue 3
Abstract
In this study physiological, certain blood hormones and immune status of male and female Broad Breasted White turkeys were recorded under cold stress. Birds were acclimated two weeks prior to the start of experiment and later divided into two groups. Control group (n=12 with equal number from each sex) was maintained at an environmental temperature of 27-30°C and Test group (n=12 with equal number from each sex) was housed in a designed chamber were the temperature (10±1°C) remained stable at least for 5-6 hours in a day. After 3 consecutive days (72 hours) of temperature treatment Phytohaemagglutinin-P (PHA-P) at the dose rate of 0.5 mg in 0.1 ml PBS were injected to the patagium of both control and test group of birds to study the cell mediated immune response by measuring the dermal swelling in response to inflammatory reaction after 24 hours of injection of Phytohaemagglutinin-P (PHAP). After 96 hours of cold treatment the blood samples were collected from the wing vein to analyze the blood hormonal levels using standard protocols. The physiological parameters like respiration rate, rectal temperature and surface temperature were recorded at the morning hours of the day during experimental period. Significant difference (p≤0.01) between treatments were observed in body temperatures, respiration rate, Heterophil%, H/L ratio, Wing web thickness, T4 and cortisol levels.
Authors and Affiliations
Ovais Aarif, P. Mahapatra, M. Yatoo, Showket Dar
Keywords
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