IN SILICO PREDICTION AND VALIDATION OF MICRORNAS FROM JAPANESE ENCEPHALITIS VIRUS (JEV)
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: MicroRNAs are endogenous, small, single stranded, non coding RNAs having 19-25 nucleotides. These miRNAs are complementary to their target messenger RNAs that bind principally to its 3' un translated regions (3’ UTRs). Small RNAs play crucial roles in the regulation of gene expression in many eukaryotes; therefore it is important to predict potential viral miRNAs which might be involved in an establishment of Japanese Encephalitis virus (JEV) disease. Different computational approaches and methods were used for predicting viral microRNAs from the JEV genome in this work.Methods: In the present study, the use of genome-wide computational approach has been demonstrated to predict miRNAs and their target(s) in JEV genome. Two freely accessible softwares, MiPred and Genscan were used to predict the secondary structures of the potential miRNAs.Results: In all, 36 miRNAs were predicted and characterized by conducting genome-wide homology search against all the reported miRNAs. These miRNAs were further validated by performing phylogenetic analyses and using statistical tools.Further, attempt was made to predict the 3′ untranslated regions of mRNAs from whole genome of JEV which may prove helpful in finding putative targets of these miRNAs.Conclusion: This is the first study to identify and validate miRNAs in JEV which is an important step in identifying putative JEV miRNAs that utilize host cell machinery, and may play a crucial role in neuroinflammation and silencing of host genes, thus demonstrating the role of viral miRNAs in establishing viral pathogenesis.Â
Authors and Affiliations
Neema Tufchi, Kumud Pant, Bhasker Pant, Syed Mohsin Waheed
Keywords
Related Articles
- EP ID EP578714
- DOI -
- Views 86
- Downloads 0
IS BACTERIOLOGY A CONTRIBUTING FACTOR IN UNSALVAGEABLE NATURE OF DIABETIC FOOT INFECTIONS?–A STUDY IN A DISTRICT HOSPITAL IN MALAYSIA
Objective: The objective is to study bacterial pathogens isolated in diabetic foot infection (DFI) and their sensitivity pattern to antibiotics commonly used in the management of DFI in the salvageable and unsalvageable...
BACTERIOCIN: A NOVEL APPROACH FOR PRESERVATION OF FOOD
Bacteriocins are antimicrobial peptides which are ribosomally synthesized and produced by Lactic acid bacteria. They play a major role in prevention of human disease such as cancer, inflammatory disease, respiratory in...
ASSAY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS QUANTITATIVE ESTIMATION OF DILOXANIDE FUROATE AND ORNIDAZOLE IN TABLETS BY RP-HPLC
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous quantitative estimation of Diloxanide furoate and Ornidazole in tablets and va...
ANTIFERTILITY ACTIVITY OF β-SITOSTEROL ISOLATED FROM BARLERIA PRIONITIS (L.) ROOTS IN MALE ALBINO RATS.
Objective: Plant–derived male antifertility agents need to address the basic requirements of fertility suppression without affecting libido. This study was undertaken to evaluate the male antifertility potential of the...
ANTI-INFLAMMATORY AND PROTECTIVE PROPERTIES OF ALOE VERA LEAF CRUDE GEL IN CARRAGEENAN INDUCED ACUTE INFLAMMATORY RAT MODELS
Objectives: Current clinical treatment regimes for inflammatory diseases have different drawbacks including side effects of the drugs and the high cost of long term treatment. In the last few decades different promising...