In silico prediction of B cell epitopes of the hemolysis-associated protein 1 for vaccine design against leptospirosis
Journal Title: Journal of Current Biomedical Reports - Year 2020, Vol 1, Issue 1
Abstract
Leptospirosis is known as a zoonotic disease of global importance originated from infection with the spirochete bacterium Leptospira. Although several leptospirosis vaccines have been tested, the vaccination is relatively unsuccessful in clinical application despite decades of research. Therefore, this study was conducted to predict B cell epitopes of the hemolysis-associated protein 1 (Hap1) for vaccine design against leptospirosis. For prediction of linear epitopes, the sequence of extracellular region of Hap1 was submitted to ABCpred, BCPREDs, Bcepred, Bepipred and Ellipro servers. DiscoTope 2.0 and B-pred servers were used for prediction of conformational epitopes from the entire PDB structure of Hap1 that obtained from the homology modeling method. Further analysis for solvent accessible areas and relative solvent accessibility of all the residues on the PDB structures using Naccess program and NetSurfP server defined that predicted conformational B cell epitopes had higher solvent accessible and their residues were exposed on the surface therefore, immunoinformatics analysis showed that hemolysis-associated protein 1 can properly stimulate the B cells immune responses.
Authors and Affiliations
Sakineh Poorhosein Fookolaee, Somayyeh Talebishelimaki, Mohammad Taha Saadati Rad, Mostafa Akbarian Rokni
Keywords
Related Articles
- EP ID EP688951
- DOI -
- Views 293
- Downloads 0
The sound of getting rid of coronavirus by RNA interference technology: RNAi against COVID-19
No Abstract.
Investigating the effect of ghrelin on improving memory and learning ability in animals: A systematic review
Ghrelin does an important role in neurological functions such as memory, learning, and cognition as well. There is some evidence that ghrelin can improve memory. This study aimed to evaluate the effects of ghrelin on rat...
Using decoction of some vitamin C enriched plants for the management of COVID-19 in Jos, Nigeria: A case report
The worldwide influence of coronavirus disease 2019 (COVID-19) pandemic compelled the global health organizations to prioritize the situation over all other related community health issues. The lack of specific treatment...
Molecular investigation of extended-spectrum β-lactamases (ESBLs) genes in the Salmonella isolates obtained from children with acute diarrhea
Salmonellosis is an important public health concern among children in worldwide. Extended-spectrum β-lactams (ESBLs) cause resistance to clinically important beta-lactams which are generally used to treat invasive Salmon...
The role of immunotherapy in eliminating high-risk treatments for pediatric cancer
Chemotherapy, radiation, and surgery are often ineffective in treating refractory or chronic tumors in children. Recent advances in cancer immunotherapy have also improved the outcome of many human tumors, resulting in s...