In Silico Study, Synthesis and Evaluation of Cytotoxic Activity of New Sulfonamide-Isatin Derivatives as Carbonic Anhydrase Enzyme Inhibitors
Journal Title: International Journal of Health & Medical Research - Year 2024, Vol 3, Issue 08
Abstract
Aim: Design, molecular docking, synthesis, and evaluation of cytotoxic activity of new compounds I, II, III, and IV that have isatin-sulfonamide derivatives. Materials and Methods: for chemical synthesis, chemical compounds such as sulfonamide, 4-amino ethyl benzoate, isatin, and its derivatives were used. For the docking study (MOE), software program version 2015.10 was used. The MTT assay was utilized to predict the cytotoxic activity. Results: The synthesized compounds demonstrated significant inhibition of carbonic anhydrase XII activity through molecular docking, as well as significant inhibition of cancer cell viability. Compounds II and IV show higher S-scores than acetazolamide. Also, the MTT assay shows that compounds II and IV have IC50 values of 0.06 µM and 0.105 µM against MCF-7 cells, respectively, while acetazolamide has an IC50 value of 0.394 µM. While acetazolamide had an IC50 of 0.901 µM, compounds II and IV had IC50s of 0.063 µM and 0.114 µM against Hct116 cells, respectively. The MTT assay explains compounds II and IV have better cytotoxic activity compared with acetazolamide. Conclusion: New compounds that were produced showed signs of cytotoxicity and carbonic anhydrase inhibitory qualities.
Authors and Affiliations
Tuqa salim Hussein, Ammar Abdul Aziz Alibeg
Keywords
Related Articles
- EP ID EP750584
- DOI 10.58806/ijhmr.2024.v3i08n15
- Views 60
- Downloads 0
Pathogenic Bacteria Associated with Cholecystitis: A Review Article
Cholecystitis is defined as an inflammation of the gallbladder. It is of major concern because if left untreated, it can progress to complex pathologies. The high burden of cholecystitis underlines the importance of epid...
Emerging Trends In Immunotherapy Management For Pancreatic Cancer: A Literature Review
The prognosis for pancreatic cancer (PC) is poor making it a substantial treatment challenge. A potentially effective treatment option is immunotherapy. To better understand the changing field of immunotherapy in PC mana...
The Biochemistry and Clinical Significance of D-Dimer
D-dimer, product degradation of fibrin, belongs to the most sensitive and specific markers in the diagnostics of thrombotic disorders, mainly acute PE and DVT. This leads to its measurement because of the high sensitivit...
Association Between Prostatitis and Some Biochemical Markers in Patients of the AL-Najaf Province, Iraq
One of the most common clinical conditions in men is prostate disease, especially prostatitis, which causes various symptoms, that negatively affect patient life. Our current study investigated the relationship between p...
Ebstein’s Anomaly Surpassing 6th Decade of Life- A Case Report
Ebstein’s anomaly (EA) is a rare congenital heart defect usually presenting around birth years, where in the septal and posterior leaflets of the tricuspid valve are displaced towards the apex of the right ventricle, atr...