In vitro alpha-glucosidase inhibitory activity of phenolic constituents from aerial parts of Polygonum hyrcanicum
Journal Title: DARU Journal of Pharmaceutical Sciences - Year 2012, Vol 20, Issue 0
Abstract
Background and the purpose of the study The early stage of diabetes mellitus type 2 is associated with postprandial hyperglycemia. Hyperglycemia is believed to increase the production of free radicals and reactive oxygen species, leading to oxidative tissue damage. In an effort of identifying herbal drugs which may become useful in the prevention or mitigation of diabetes, biochemical activities of Polygonum hyrcanicum and its constituents were studied. Methods Hexane, ethylacetate and methanol extracts of P. hyrcanicum were tested for alpha-glucosidase inhibitory, antioxidant and radical scavenging properties. Active constituents were isolated and identified from the methanolic extract in an activity guided approach. Results A methanolic extract from flowering aerial parts of the plant showed notable alpha-glucosidase inhibitory activity (IC50 = 15 mug/ml). Thirteen phenolic compounds involving a cinnamoylphenethyl amide, two flavans, and ten flavonols and flavonol 3-O-glycosides were subsequently isolated from the extract. All constituents showed inhibitory activities while compounds 3, 8 and 11 (IC50 = 0.3, 1.0, and 0.6 muM, respectively) were the most potent ones. The methanol extract also showed antioxidant activities in DPPH (IC50 = 76 mug/ml) and FRAP assays (1.4 mmol ferrous ion equivalent/g extract). A total phenol content of 130 mg/g of the extract was determined by Folin-Ciocalteu reagent. Conclusion This study shows that P. hyrcanicum contains phenolic compounds with in vitro activity that can be useful in the context of preventing or mitigating cellular damages linked to diabetic conditions.
Authors and Affiliations
F Moradi-Afrapoli, M Mirjani, Abbas Hadjiakhoondi, Narguess Yassa, Peyman Salehi, Behavar Asghari, Mobina Malmir, Soodabeh Saeidnia, Yusef Ajani, Reza Dolatabadi, Mattias Hamburger
Keywords
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