In vitro Anti-erythrocyte Sickling Effect of Lunularic Acid of Natural Origin
Journal Title: International Blood Research & Reviews - Year 2015, Vol 4, Issue 3
Abstract
Aims: To evaluate the antisickling activity of lunularic acid. Study Design: Biological experiment in vitro (Emmet test), evaluation of cell morphological parameters such as radius, perimeter and surface. Place and Duration of Study: University of Kinshasa, Democratic Republic of the Congo, from February 2015 to June 2015. Methodology: The antisickling activity is carried out in vitro in isotonic (NaCl 0.9%) and hypoxic conditions (Na2S2O5 2%) using Sickle red blood cells (RBCs) as model system. The RBCs phenotype were analyzed using a computer assisted image analysis program (Motic Images 2000, version 1.3; Motic China Group Co LTD) and statistical data analyses were processed using Microcal Origin 8.5 Pro package software. Results: The biological testing revealed that lunularic acid has antisickling activity (Normalization rate > 95% at 25 µg/ml). This phenolic acid acts by reducing the perimeter of sickle RBCs and by increasing their surface. The treated SS RBCs demonstrated a remarkable similarity to normal blood cells values of morphological parameters. Conclusion: The naturally occurring lunularic acid could serve as promising lead compound candidate for improving the quality life of SCD patients.
Authors and Affiliations
K. N. Ngbolua, Rafatro Herintsoa, Rakotoarimanana Hajatiana, V. Mudogo, D. D. Tshilanda, P. T. Mpiana
Keywords
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- EP ID EP242613
- DOI 10.9734/IBRR/2015/21718
- Views 123
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