In vitro Anti-inflammatory and Protective Effects of ibidì® on Intestinal Epithelial Cells
Journal Title: European Journal of Medicinal Plants - Year 2014, Vol 4, Issue 9
Abstract
Aim: To define the putative anti-inflammatory and cytotoxic effects of ibidì®, a new phytotherapeutic formulation composed of three extracts: Punica granatum L, pericarpum; Boswellia serrata Roxb., resina; Curcuma longa L,. rhizome, using Caco-2 cells, an in vitro model of human intestinal epithelium. Methodology: cytotoxicity and capacity of ibidì® to induce cell proliferation were assessed respectively by 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay and nucleotide 5-bromo-2’-deoxyuridine (BrdU) incorporation. Cell migration was evaluated by scratch wound assay. COX-2, IL-6, IL-8 and MCP-1 protein levels were measured in the supernatant of cells stimulated with or without TNF-alfa or IL-1 beta in presence or in absence of ibidì® using ELISA assays. Finally, the influence of ibidì® on the integrity, paracellular permeability, and viability of Caco-2 cell monolayers was monitored by measuring the transepithelial electrical resistance (TEER) in presence or in absence of TNF- stimulation. Results: No dose-response toxicity was observed after 48 h incubation with ibidì®. Interestingly the cell proliferation rate was generally lower in presence of ibidì® than vehicle at all concentrations tested, while ibidì® had no effects on cell migration. Ibidì® markedly inhibited TNF-alfa-induced production of IL-8 at all concentrations tested in a dose-response manner, while that of IL-6 and MCP-1 only at highest ibidì® concentrations. Importantly ibidì® in a range of concentration between 145 and 9 µg/ml not only abrogated TNF-alfa-dependent TEER depression, but also promoted higher resistance values than untreated cells. Conclusion: These data demonstrate that ibidì® exerts anti-inflammatory and protective effects on intestinal epithelial cells by blocking the production of IL-8, IL-6 and MCP-1, and unveil that the synergism of the three extracts regulates epithelial barrier function.
Authors and Affiliations
Luciana Petti, Francesca De Santis, Stefania Vetrano
Keywords
Related Articles
- EP ID EP350113
- DOI 10.9734/EJMP/2014/11018
- Views 118
- Downloads 0
Quantification of Antinociceptive and Anti-inflammatory Potentials of Different Ocimum gratissimum Linn. Leaf Extracts in Male Whistar Albino Rats
Aim: This study was aimed at quantifying the antinociceptive and anti-inflammatory potency and efficacy of aqueous (AQ) and ethanolic leaf extracts of O. gratissimum (ET) in Whistar rats. Methodology: Inflammation (paw...
An Evaluation of the Preventive Effect of the Methanol Extract of Thai Basil (Ocimum basilicum L.) on Ethanol-induced Hepatotoxicity in Rats
Aim: The present study was investigated the preventive effect of methanol extract of Ocimum bacilicum (MOEB) on ethanol induced hepatotoxicity in rats. Study Design: Male Wistar rats weighing 200–250 g were divided into...
The Effect of Hydroalcoholic Extract of Ferulago angulata on Liver Function Parameters and Antioxidant Status in Alloxan-Induced Diabetic Rats
Aim: The aim of this study was to evaluate the hepatoprotective and antioxidant activities of Ferulago angulata Extract (FAE) in experimental diabetic rats. Materials and Methods: 54 adult male Wistar rats divided into 6...
Anti-Allergic Action of Aqueous Extract of Moringa oleifera Lam. Leaves in Mice
Aims: The present investigation aimed to evaluate anti-allergic activity of an aqueous extract of Moringa oleifera Lam. leaves (AqMOL) in three type I allergy models in mice. Study Design: Anti-allergic assay of AqMOL...
An Evaluation of the Sub Acute Toxicity and Haemostatic Effects of Leaves Extract of Achyranthes aspera in Mice and Albino Rats
Background: Achyranthes aspera is used mostly in Nigerian and other folklore medicines to stop bleeding from wounds and in the treatment of other conditions such as dog bite, scorpion bite, gonorrhoea, obstetric disorder...