IN VITRO ANTIPLASMODIAL AND CYTOTOXIC ACTIVITIES OF A SUNGKAI (PERONEMA CANESCENS) LEAF EXTRACT

Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 10

Abstract

Objective: The aim of this study was to assess the antiplasmodial and cytotoxic activities and to evaluate the selectivity indices of acetone, ethanol and aqueous extracts of Peronema canescens leaves.Methods: Antiplasmodial activity was measured in vitro against Plasmodium falciparum strains D10 and FCR3 by 72 h incubation at 37 °C in a candle jar. Parasitaemia was calculated by counting the parasite numbers in thin smears. In vitro cytotoxicity was assayed in Vero cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and reading the absorbency at 595 nm with an ELISA reader. The assessed parameters included: 50% inhibitory concentration (IC50) of antiplasmodial activity, IC50 of cytotoxic activity and the selectivity index of the Peronema canescens leaf extract.Results: The IC50 values for the acetone, ethanol and aqueous extracts were 26.33±1.65, 37.96±8.17 and 12.26±1.05 μg/ml, respectively, against the Plasmodium falciparum D10 strain and 51.14±8.65, 70.22±14.13 and 34.85±6.04 μg/ml, respectively, against the FCR3 strain. For Vero cells, the IC50 values for the acetone, ethanol and aqueous extracts were 23.37±5.63, 629.46±24.85 and 634.00±144.82 μg/ml, respectively. The selectivity indices of these extracts were 0.89, 16.46 and 51.70, respectively, for the D10 strain and 0.46, 8.90 and 18.00, respectively, for the FCR3 strain.Conclusion: The aqueous extract of Peronema canescens leaves had the highest in vitro antiplasmodial activity and the best selectivity index.

Authors and Affiliations

Jhons Fatriyadi Suwandi, Mahardika Agus Wijayanti, Mustofa .

Keywords

Related Articles

SOLVENT EFFECT ON PHOTOSTABILITY OF BUTYL METHOXY DI BENZOYL METHANE FORMULATED IN SOLUTION AND EMULSION

Objective: The current study was undertaken to correlate the impact of some cosmetics solvents in the photostability of butyl methoxy dibenzoyl methane (BMDBM) into systems with an increasing order of complexity, from si...

EVALUATION OF IN VITRO ANTICANCER POTENTIAL OF ETHANOLIC EXTRACT AND ITS DIFFERENT FRACTIONS OF CAESALPINIA BONDUC (L) ROXB SEEDS

Objective: The present study aims to evaluate the anticancer potential of ethanolic extract and its different fractions of Caesalpinia bonduc seeds against Ehrlich Ascites Carcinoma (EAC) cell lines.Methods: Ethanolic ex...

EVALUATION OF IMMUNOMODULATORY ACTIVITY OF EXTRACT FROM RIND OF NEPHELIUM LAPPACEUM FRUIT

Objective: Nephelium lappaceum Linn (Sapindaceae), also known as Rambutan is a tropical fruit native to Malaysia. The rind of Rambutan, which is normally discarded, has been used in the present research work to explore t...

OXIDATIVE STRESS-BASED HEPATOTOXICITY OF DULOXETINE IN WISTAR RATS

Objective: Duloxetine, a selective serotonin and noradrenaline reuptake inhibitor used in major depressive disorders, urinary incontinence and diabetic peripheral neuropathic pain. It is reported to be associated with se...

NEW ONSET OF DIABETES MELLITUS IN INDIAN RENAL TRANSPLANT RECIPIENT-A RETROSPECTIVE STUDY

Objective: To identify incidence and determinant of new-onset of diabetes after transplant (NODAT) in Indian renal transplant recipients.Methods: In this study Indian renal transplant recipients who were not diabetic bef...

Download PDF file
  • EP ID EP566137
  • DOI 10.22159/ijpps.2018v10i10.25124
  • Views 79
  • Downloads 0

How To Cite

Jhons Fatriyadi Suwandi, Mahardika Agus Wijayanti, Mustofa . (2018). IN VITRO ANTIPLASMODIAL AND CYTOTOXIC ACTIVITIES OF A SUNGKAI (PERONEMA CANESCENS) LEAF EXTRACT. International Journal of Pharmacy and Pharmaceutical Sciences, 10(10), 109-113. https://www.europub.co.uk/articles/-A-566137