IN VITRO ANTIPLASMODIAL AND CYTOTOXIC ACTIVITIES OF A SUNGKAI (PERONEMA CANESCENS) LEAF EXTRACT
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 10
Abstract
Objective: The aim of this study was to assess the antiplasmodial and cytotoxic activities and to evaluate the selectivity indices of acetone, ethanol and aqueous extracts of Peronema canescens leaves.Methods: Antiplasmodial activity was measured in vitro against Plasmodium falciparum strains D10 and FCR3 by 72 h incubation at 37 °C in a candle jar. Parasitaemia was calculated by counting the parasite numbers in thin smears. In vitro cytotoxicity was assayed in Vero cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and reading the absorbency at 595 nm with an ELISA reader. The assessed parameters included: 50% inhibitory concentration (IC50) of antiplasmodial activity, IC50 of cytotoxic activity and the selectivity index of the Peronema canescens leaf extract.Results: The IC50 values for the acetone, ethanol and aqueous extracts were 26.33±1.65, 37.96±8.17 and 12.26±1.05 μg/ml, respectively, against the Plasmodium falciparum D10 strain and 51.14±8.65, 70.22±14.13 and 34.85±6.04 μg/ml, respectively, against the FCR3 strain. For Vero cells, the IC50 values for the acetone, ethanol and aqueous extracts were 23.37±5.63, 629.46±24.85 and 634.00±144.82 μg/ml, respectively. The selectivity indices of these extracts were 0.89, 16.46 and 51.70, respectively, for the D10 strain and 0.46, 8.90 and 18.00, respectively, for the FCR3 strain.Conclusion: The aqueous extract of Peronema canescens leaves had the highest in vitro antiplasmodial activity and the best selectivity index.
Authors and Affiliations
Jhons Fatriyadi Suwandi, Mahardika Agus Wijayanti, Mustofa .
Keywords
Related Articles
- EP ID EP566137
- DOI 10.22159/ijpps.2018v10i10.25124
- Views 80
- Downloads 0
TOXICITY OF TAGETES MINUTA ESSENTIAL OIL IN SILVER CATFISH (RHAMDIA QUELEN)
Objective: Evaluation of Tagetes minuta L. essential oil (EOTM) toxicity in the silver catfish Rhamdia quelen.Methods: The EOTM extracted by hydrodistillation was identified qualitatively based on retention indices and t...
IN VITRO EVALUATION OF IBUPROFEN HOT-MELT EXTRUDED PELLETS EMPLOYING DIFFERENT DESIGNS OF THE FLOW THROUGH CELL
Objective: Hot-melt extrusion technique (HME) was used to prepare a sustained release (SR) multiparticulate oral dosage form (pellets) containing Ibuprofen (IBU). Prepared IBU-HME pellets were in vitro evaluated by flow-...
EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS
Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The p...
PHARMACOGNOSTICAL, PHYTOCHEMICAL SCREENING AND STANDARDIZATION OF NELUMBO NUCIFERA (GAERTN): THE SACRED LOTUS
Objective: To perform the Phytochemical screening & standardization of Nelumbo nucifera Gaertn.Methods: The present study provides pharmacognostic, physicochemical and phytochemicals details of Parts of Nelumbo nucif...
DETERMINATION OF ANTIBACTERIAL, ANTIFUNGAL, BIOACTIVE CONSTITUENTS OF TRIPHALA BY FT-IR AND GC-MS ANALYSIS
Objectives: To characterize the number of phytoconstituents present in triphala using FT-IR and GC-MS.Methods: Antibacterial activity was measured by disc diffusion method, antifungal activity were analyzed by poisoned f...