In Vitro Caco-2 Cell Permeability Studies of Ziprasidone Hydrochloride Monohydrate Nanocrystals
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2021, Vol 18, Issue 2
Abstract
Objectives: The current study focused on the evaluation of the cytotoxic effect and permeability of ziprasidone hydrochloride monohydrate (ZHM) nanocrystals on Caco-2 cells. Materials and Methods: ZHM nanocrystals were prepared by the microfluidization method in the presence of polyvinylpyrrolidone as a stabilizer. Particle size (PS), particle size distribution (PDI), and zeta potential (ZP) values were measured in characterization studies. In vitro cytotoxic effects of ZHM nanocrystals were investigated using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. Caco-2 transport studies were conducted with formulations of ZHM coarse powder and nanocrystals. Results: Nanocrystals were obtained with 400-600 nm PS, 0.1-0.4 PDI, and >20 mV ZP values. The cell viability remained 100% for all sample groups. The permeability value of ZHM nanocrystals through Caco-2 cells increased 2.3-fold in comparison with ZHM coarse powder. Cumulative drug transport also increased at the end of the sampling period. Conclusion: Nanocrystal technology helps to increase the permeability of drug particles by increasing the saturation solubility.
Authors and Affiliations
Alptuğ KARAKÜÇÜK, Emine TAŞHAN, Naile ÖZTÜRK, Nevin ÇELEBİ
Keywords
Related Articles
- EP ID EP694451
- DOI 10.4274/tjps.galenos.2020.67366
- Views 90
- Downloads 0
Degradation Kinetics, In Vitro Dissolution Studies, and Quantification of Praziquantel, Anchored in Emission Intensity by Spectrofluorimetry
Objectives: A simple, rapid, specific, and highly sensitive ecofriendly spectrofluorimetric method has been developed for the quantification of praziquantel. Materials and Methods: A linear relationship was found betwee...
Comparison of Lipid and Lipoprotein Values of Wrestlers and Soccer Players
Objectives: The aim of this study was to compare the lipid and lipoprotein values of wrestlers and soccer players. Materials and Methods: A total of 35 subjects, 17 male wrestlers who are sporting for 11.5 years and 18...
Floating Microspheres of Enalapril Maleate as a Developed Controlled Release Dosage Form: Investigation of the Effect of an Ionotropic Gelation Technique
Objectives: The purpose of this study was to provide a control drug delivery system through a newly approved work to enhance the absorption and bioavailability of enalapril maleate loaded floating microspheres by ionotro...
Local Use of Apisan Gel, A New Oral Care Product in the Treatment of Experimental Periodontitis Against the Background of Hyperacid Gastritis and Intoxication with Tobacco Smoke
Objectives: The aim of the work was to substantiate the use of a newly created oral care product in the treatment of periodontal disease reconstructed against the background of hyperacidic gastritis under the conditions...
Theoretical Study on Ionization of Boric Acid in Aqueous Solution by Ab Initio and DFT Methods at T=298.15 K
Objectives: The aim of this research work was to theoretically calculate the pKa value of boric acid in aqueous solution by theoretical methods at T=298.15 K. Materials and Methods: Boric acid has antifungal and antivir...